WFRF:(Shashkova Sviatlana 1987)
Sökning: WFRF:(Shashkova Sviatlana 1987) > Correlating single-...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04068naa a2200589 4500 | |
| 001 | oai:research.chalmers.se:fe66fb4f-db8e-4d55-bea6-71dc27e4c658 | |
| 003 | SwePub | |
| 008 | 201223s2021 | |||||||||||000 ||eng| | |
| 009 | oai:gup.ub.gu.se/294281 | |
| 024 | 7 | a https://research.chalmers.se/publication/5212712 URI |
| 024 | 7 | a https://doi.org/10.1016/j.ymeth.2020.05.0032 DOI |
| 024 | 7 | a https://gup.ub.gu.se/publication/2942812 URI |
| 040 | a (SwePub)cthd (SwePub)gu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Shashkova, Sviatlana,d 1987u University of York4 aut0 (Swepub:gu)xshasv |
| 245 | 1 0 | a Correlating single-molecule characteristics of the yeast aquaglyceroporin Fps1 with environmental perturbations directly in living cells |
| 264 | 1 | b Elsevier BV,c 2021 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Membrane proteins play key roles at the interface between the cell and its environment by mediating selective import and export of molecules via plasma membrane channels. Despite a multitude of studies on transmembrane channels, understanding of their dynamics directly within living systems is limited. To address this, we correlated molecular scale information from living cells with real time changes to their microenvironment. We employed super-resolved millisecond fluorescence microscopy with a single-molecule sensitivity, to track labelled molecules of interest in real time. We use as example the aquaglyceroporin Fps1 in the yeast Saccharomyces cerevisiae to dissect and correlate its stoichiometry and molecular turnover kinetics with various extracellular conditions. We show that Fps1 resides in multi tetrameric clusters while hyperosmotic and oxidative stress conditions cause Fps1 reorganization. Moreover, we demonstrate that rapid exposure to hydrogen peroxide causes Fps1 degradation. In this way we shed new light on aspects of architecture and dynamics of glycerol-permeable plasma membrane channels. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Cellbiologi0 (SwePub)106042 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Cell Biology0 (SwePub)106042 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
| 653 | a Single-molecule | |
| 653 | a Plasma membrane channels | |
| 653 | a Living cell | |
| 653 | a Osmotic condition | |
| 653 | a Super-resolution | |
| 653 | a Cell stress | |
| 653 | a Cell stress | |
| 653 | a Living cell | |
| 653 | a Osmotic condition | |
| 653 | a Plasma membrane channels | |
| 653 | a Single-molecule | |
| 653 | a Super-resolution | |
| 700 | 1 | a Andersson, Mikaelu Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology4 aut0 (Swepub:gu)xanmik |
| 700 | 1 | a Hohmann, Stefan,d 1956u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:gu)xhohst |
| 700 | 1 | a Leake, Mark C.u University of York4 aut |
| 710 | 2 | a University of Yorkb Institutionen för kemi och molekylärbiologi4 org |
| 773 | 0 | t Methodsd : Elsevier BVg 193, s. 46-53q 193<46-53x 1095-9130x 1046-2023 |
| 856 | 4 | u https://research.chalmers.se/publication/521271/file/521271_Fulltext.pdfx primaryx freey FULLTEXT |
| 856 | 4 | u https://doi.org/10.1016/j.ymeth.2020.05.003 |
| 856 | 4 8 | u https://research.chalmers.se/publication/521271 |
| 856 | 4 8 | u https://doi.org/10.1016/j.ymeth.2020.05.003 |
| 856 | 4 8 | u https://gup.ub.gu.se/publication/294281 |
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