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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003346naa a2200445 4500
001oai:DiVA.org:kth-235174
003SwePub
008180917s2018 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2351742 URI
024a https://doi.org/10.1021/acssynbio.8b000562 DOI
040 a (SwePub)kth
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Shabestary, Kiyanu KTH,Systembiologi4 aut0 (Swepub:kth)u1a0fac0
2451 0a Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria
264 c 2018-06-06
264 1b American Chemical Society (ACS),c 2018
338 a electronic2 rdacarrier
500 a QC 20180920
520 a Photoautotrophic production of fuels and chemicals by cyanobacteria typically gives lower volumetric productivities and titers than heterotrophic production. Cyanobacteria cultures become light limited above an optimal cell density, so that this substrate is not supplied to all cells sufficiently. Here, we investigate genetic strategies for a two-phase cultivation, where biofuel-producing Synechocystis cultures are limited to an optimal cell density through inducible CRISPR interference (CRISPRi) repression of cell growth. Fixed CO2 is diverted to ethanol or n-butanol. Among the most successful strategies was partial repression of citrate synthase gltA. Strong repression (>90%) of gitA at low culture densities increased carbon partitioning to n-butanol 5-fold relative to a nonrepression strain, but sacrificed volumetric productivity due to severe growth restriction. CO2 fixation continued for at least 3 days after growth was arrested. By targeting sgRNAs to different regions of the gitA gene, we could modulate GItA expression and carbon partitioning between growth and product to increase both specific and volumetric productivity. These growth arrest strategies can be useful for improving performance of other photoautotrophic processes.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653 a cyanobacteria
653 a CRISPRi
653 a bioproduction
653 a Bioteknologi
653 a Biotechnology
700a Anfelt, Josefinu KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1035z36
700a Ljungqvist, Emilu KTH4 aut0 (Swepub:kth)u1qfloha
700a Jahn, Michaelu KTH,Systembiologi4 aut0 (Swepub:kth)u1ryh6dw
700a Yao, Lunu KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1nych9u
700a Hudson, Elton P.u KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1ja6ik3
710a KTHb Systembiologi4 org
773t ACS Synthetic Biologyd : American Chemical Society (ACS)g 7:7q 7:7x 2161-5063
856u https://kth.diva-portal.org/smash/get/diva2:1248671/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint
856u http://kth.diva-portal.org/smash/get/diva2:1248671/FULLTEXT01
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-235174
8564 8u https://doi.org/10.1021/acssynbio.8b00056

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