L773:1096 7176 OR L773:1096 7184
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Coupled incremental precursor and co-factor supply improves 3-hydroxypropionic acid production in Saccharomyces cerevisiae
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- Chen, Yun, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bao, Jichen, 1988 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kim, Il-Kwon, 1969 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Elsevier BV, 2014
- 2014
- English.
- In: Metabolic Engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 22, s. 104-109
- Journal article (peer-reviewed)
Abstract
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- 3-Hydroxypropionic acid (3-HP) is an attractive platform chemical, which can be used to produce a variety of commodity chemicals, such as acrylic acid and acrylamide. For enabling a sustainable alternative to petrochemicals as the feedstock for these commercially important chemicals, fermentative production of 3-HP is widely investigated and is centered on bacterial systems in most cases. However, bacteria present certain drawbacks for large-scale organic acid production. In this study, we have evaluated the production of 3-HP in the budding yeast Saccharomyces cerevisiae through a route from malonyl-CoA, because this allows performing the fermentation at low pH thus making the overall process cheaper. We have further engineered the host strain by increasing availability of the precursor malonyl-CoA and by coupling the production with increased NADPH supply we were able to substantially improve 3-HP production by five-fold, up to a final titer of 463 mg l(-1). Our work thus led to a demonstration of 3-HP production in yeast via the malonyl-CoA pathway, and this opens for the use of yeast as a cell factory for production of bio-based 3-HP and derived acrylates in the future. (C) 2014 International Metabolic Engineering Society.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
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- ref (subject category)
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