Coherent anti-Stokes Raman scattering microscopy of human smooth muscle cells in bioengineered tissue scaffolds

Brackmann, Christian, 1973 (author): Chalmers University of Technology, Molecular Microscopy, Department of Chemical and Biological Engineering, Göteborg, Sweden,Chalmers tekniska högskola

Esguerra, Maricris, 1981 (author): Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery,Sahlgrenska Academy at University of Gothenburg, Institute of Clinical Sciences, Department of Surgery, Göteborg, Sweden

Olausson, Daniel (author): Sahlgrenska Academy at University of Gothenburg, Institute of Clinical Sciences, Department of Surgery, Göteborg, Sweden,Göteborgs universitet,University of Gothenburg

Delbro, Dick, 1950 (author): Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery,Sahlgrenska Academy at University of Gothenburg, Institute of Clinical Sciences, Department of Surgery, Göteborg, Sweden

Krettek, Alexandra, 1968- (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,Sahlgrenska Academy at University of Gothenburg, Institute of Medicine, Department of Internal Medicine, Göteborg, Sweden

Gatenholm, Paul, 1956 (author): Chalmers University of Technology, Polymer Science, Department of Chemical and Biological Engineering, Göteborg, Sweden,Chalmers tekniska högskola

Enejder, Annika, 1969 (author): Chalmers University of Technology, Molecular Microscopy, Department of Chemical and Biological Engineering, Göteborg, Sweden,Chalmers tekniska högskola

(creator_code:org_t)

SPIE - International Society for Optical Engineering, 2011
2011
English.
In: Journal of Biomedical Optics. - : SPIE - International Society for Optical Engineering. - 1083-3668 .- 1560-2281. ; 16:2

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Abstract Subject headings

The integration of living, human smooth muscle cells in biosynthesized cellulose scaffolds was monitored by nonlinear microscopy toward contractile artificial blood vessels. Combined coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy was applied for studies of the cell interaction with the biopolymer network. CARS microscopy probing CH(2)-groups at 2845 cm(-1) permitted three-dimensional imaging of the cells with high contrast for lipid-rich intracellular structures. SHG microscopy visualized the fibers of the cellulose scaffold, together with a small signal obtained from the cytoplasmic myosin of the muscle cells. From the overlay images we conclude a close interaction between cells and cellulose fibers. We followed the cell migration into the three-dimensional structure, illustrating that while the cells submerge into the scaffold they extrude filopodia on top of the surface. A comparison between compact and porous scaffolds reveals a migration depth of <10 μm for the former, whereas the porous type shows cells further submerged into the cellulose. Thus, the scaffold architecture determines the degree of cell integration. We conclude that the unique ability of nonlinear microscopy to visualize the three-dimensional composition of living, soft matter makes it an ideal instrument within tissue engineering.

By the author/editor: Brackmann, Chris ...; Esguerra, Maricr ...; Olausson, Daniel; Delbro, Dick, 19 ...; Krettek, Alexand ...; Gatenholm, Paul, ...; show more...; Enejder, Annika, ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

By the university: University of Skövde; Chalmers University of Technology; University of Gothenburg

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