Sökning: WFRF:(Bruhn Sören) > Ulva fenestrata pro...
Fältnamn | Indikatorer | Metadata |
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000 | 05497naa a2200661 4500 | |
001 | oai:gup.ub.gu.se/307412 | |
003 | SwePub | |
008 | 240528s2021 | |||||||||||000 ||eng| | |
009 | oai:research.chalmers.se:b2f028d8-c84f-4a39-b107-f0f41d33acb1 | |
024 | 7 | a https://gup.ub.gu.se/publication/3074122 URI |
024 | 7 | a https://doi.org/10.1016/j.algal.2021.1024962 DOI |
024 | 7 | a https://research.chalmers.se/publication/5263132 URI |
040 | a (SwePub)gud (SwePub)cth | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Juul, Louiseu Århus Universitet,Aarhus University4 aut |
245 | 1 0 | a Ulva fenestrata protein – comparison of three extraction methods with respect to protein yield and protein quality |
264 | 1 | b Elsevier BV,c 2021 |
520 | a Seaweed is gaining attention as a possible alternative and sustainable source of proteins. This study investigates three protein extraction methods and their effect on protein yield and quality when applied to Ulva fenestrata . Two of the methods included alkaline extractions (pH-shifts); one version solubilizing the proteins at pH 8.5 and one solubilizing them at pH 8.5 followed by pH 12 (pH 8.5+12). The third method was a mechanical pressing, using a double screw press. All extraction methods were followed by isoelectric precipitation to concentrate the proteins. Extraction at pH 8.5 gave the significantly highest total protein yield after the isoelectric precipitation, followed by extraction at pH 8.5+12 and lastly mechanical extraction gave the lowest yield. Proteins extracted with both alkaline methods had a significantly higher solubility at pH 7 and pH 9, compared to proteins from the mechanical pressing. There were no significant differences between the three methods in total D/L-amino acid ratio. Amino acid cross-links measured as lysinoalanine (LAL) and lanthionine (LAN) where found in significantly higher amounts in alkali-extracted proteins compared to mechanically extracted, however not to a degree that expect to compromise functional or nutritional quality. Further, no significant difference in protein in vitro digestibility was found between extraction methods. In conclusion, results indicated that protein extraction at pH 8.5 can be recommended, especially regarding total protein yield and solubility of the final protein extract. | |
650 | 7 | a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Naturresursteknik0 (SwePub)2072 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Environmental Engineering0 (SwePub)2072 hsv//eng |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Miljöbioteknik0 (SwePub)2082 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Environmental Biotechnology0 (SwePub)2082 hsv//eng |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknik0 (SwePub)2092 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnology0 (SwePub)2092 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Andra medicinska och farmaceutiska grundvetenskaper0 (SwePub)301992 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Other Basic Medicine0 (SwePub)301992 hsv//eng |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Industriell bioteknikx Annan industriell bioteknik0 (SwePub)209992 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Industrial Biotechnologyx Other Industrial Biotechnology0 (SwePub)209992 hsv//eng |
653 | a Seaweed | |
653 | a protein extraction | |
653 | a pH-shift | |
653 | a mechanical pressing | |
653 | a digestibility | |
653 | a process-induced modifications | |
653 | a Seaweed | |
700 | 1 | a Danielsen, Marianneu Århus Universitet,Aarhus University4 aut |
700 | 1 | a Nebel, Carolineu Århus Universitet,Aarhus University4 aut |
700 | 1 | a Steinhagen, Sophieu Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Institutionen för marina vetenskaper, Tjärnö marinlaboratoriet,SWEMARC,Department of marine sciences,Department of marine sciences, Tjärnö Marine Laboratory,University of Gothenburg,Århus Universitet,Aarhus University4 aut0 (Swepub:gu)xstsop |
700 | 1 | a Bruhn, Annetteu Århus Universitet,Aarhus University4 aut |
700 | 1 | a Krogh Jensen, Søren4 aut |
700 | 1 | a Undeland, Ingrid,d 1968u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)undeland |
700 | 1 | a Kastrup Dalsgaard, Trineu Århus Universitet,Aarhus University4 aut |
710 | 2 | a Århus Universitetb Institutionen för marina vetenskaper4 org |
773 | 0 | t Algal Researchd : Elsevier BVg 60q 60x 2211-9264 |
856 | 4 | u https://doi.org/10.1016/j.algal.2021.102496 |
856 | 4 | u https://research.chalmers.se/publication/526313/file/526313_Fulltext.pdfx primaryx freey FULLTEXT |
856 | 4 8 | u https://gup.ub.gu.se/publication/307412 |
856 | 4 8 | u https://doi.org/10.1016/j.algal.2021.102496 |
856 | 4 8 | u https://research.chalmers.se/publication/526313 |
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