Sökning: WFRF:(Nakamura Masato) > Small Extracellular...
Fältnamn | Indikatorer | Metadata |
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000 | 04876naa a2200541 4500 | |
001 | oai:lup.lub.lu.se:ecb73398-ed17-45da-9b8f-9acdfb8dab85 | |
003 | SwePub | |
008 | 231204s2023 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/ecb73398-ed17-45da-9b8f-9acdfb8dab852 URI |
024 | 7 | a https://doi.org/10.1016/j.bbrc.2023.09.0222 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Suzuki, Hironoriu National Cancer Center Research Institute, Japan,Nagoya University4 aut |
245 | 1 0 | a Small Extracellular Vesicles from adipose-derived stem cells suppress cell proliferation by delivering the let-7 family of microRNAs in ovarian cancer |
264 | 1 | c 2023 |
300 | a 9 s. | |
520 | a Introduction: Ovarian cancer is the leading cause of death among women with gynecological cancer, and novel treatment options are urgently needed. Extracellular vesicles (EVs), including exosomes, may be one of the most promising therapeutic tools for various diseases. In this study, we aimed to investigate the therapeutic effects of adipose-derived stem cell-derived EVs (ADSC-EVs) on ovarian cancer cell lines. Materials and methods: ADSCs and the ovarian cancer cell lines SKOV3 and OV90 were used for analysis. ADSC-EVs were isolated through ultracentrifugation and validated using a cryotransmission electron microscope, nanoparticle tracking analysis, and western blotting. Then, the effect of ADSC-EVs on ovarian cancer cells was investigated using IncuCyte and microRNA sequencing. Moreover, the potential functions of miRNAs were evaluated by gain-of function analysis and in silico analysis. Results: ADSC-EVs suppressed SKOV3 and OV90 cell proliferation. In particular, small EVs (sEVs) from ADSCs exhibited a stronger antitumor effect than ADSC-medium/large EVs (m/lEVs). Comparison of the miRNA profiles between ADSC-sEVs and ADSC-m/lEVs, along with downstream pathway analysis, suggested the involvement of the let-7 family. Overexpression of hsa-let-7b-5p and hsa-let-7e-5p significantly suppressed the proliferation of SKOV3 cells. In silico analysis revealed that four potential target genes of hsa-let-7b-5p and hsa-let-7e-5p were significantly associated with the prognoses of the patients. Conclusion: ADSC-sEVs had a stronger antitumor effect than ADSC-m/lEVs. Hsa-let-7b-5p and hsa-let-7e-5p, which are highly abundant in ADSC-sEVs, suppressed cell proliferation. These findings may open up new possibilities for therapeutic approaches using ADSC-sEVs. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
653 | a hsa-let-7b-5p | |
653 | a hsa-let-7e-5p | |
653 | a Medium/large extracellular vesicles | |
653 | a Ovarian cancer | |
653 | a Small extracellular vesicles | |
700 | 1 | a Yokoi, Akirau Nagoya University4 aut |
700 | 1 | a Uno, Kanameu Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancercellers evolution,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine,Pathways of cancer cell evolution,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Nagoya University4 aut0 (Swepub:lu)ka6202un |
700 | 1 | a Yoshida, Kosukeu National Cancer Center Research Institute, Japan,Nagoya University4 aut |
700 | 1 | a Kitagawa, Masamiu Nagoya University4 aut |
700 | 1 | a Asano-Inami, Eriu Nagoya University4 aut |
700 | 1 | a Matsuo, Seikou Nagoya University4 aut |
700 | 1 | a Nagao, Yukariu Nagoya University4 aut |
700 | 1 | a Suzuki, Kazuhirou Nagoya University4 aut |
700 | 1 | a Nakamura, Kaeu Nagoya University4 aut |
700 | 1 | a Yoshihara, Masatou Nagoya University4 aut |
700 | 1 | a Tamauchi, Satoshiu Nagoya University4 aut |
700 | 1 | a Shimizu, Yusukeu Nagoya University4 aut |
700 | 1 | a Ikeda, Yoshikiu Nagoya University4 aut |
700 | 1 | a Yoshikawa, Nobuhisau Nagoya University4 aut |
700 | 1 | a Kajiyama, Hiroakiu Nagoya University4 aut |
700 | 1 | a Yamamoto, Yusukeu National Cancer Center Research Institute, Japan4 aut |
710 | 2 | a National Cancer Center Research Institute, Japanb Nagoya University4 org |
773 | 0 | t Biochemical and Biophysical Research Communicationsg 680, s. 211-219q 680<211-219x 0006-291X |
856 | 4 | u http://dx.doi.org/10.1016/j.bbrc.2023.09.022y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/ecb73398-ed17-45da-9b8f-9acdfb8dab85 |
856 | 4 8 | u https://doi.org/10.1016/j.bbrc.2023.09.022 |
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