Sökning: WFRF:(Woessmann Jakob) > Addressing the Prot...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03840naa a2200757 4500 | |
001 | oai:DiVA.org:kth-327264 | |
003 | SwePub | |
008 | 230524s2022 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3272642 URI |
024 | 7 | a https://doi.org/10.1021/acs.jproteome.2c004912 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Woessmann, Jakobu KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap4 aut0 (Swepub:kth)PI000000 |
245 | 1 0 | a Addressing the Protease Bias in Quantitative Proteomics |
264 | c 2022-08-31 | |
264 | 1 | b American Chemical Society (ACS),c 2022 |
338 | a print2 rdacarrier | |
500 | a QC 20230524 | |
520 | a Protein quantification strategies using multiple proteases have been shown to deliver poor interprotease accuracy in label-free mass spectrometry experiments. By utilizing six different proteases with different cleavage sites, this study explores the protease bias and its effect on accuracy and precision by using recombinant protein standards. We established 557 SRM assays, using a recombinant protein standard resource, toward 10 proteins in human plasma and determined their concentration with multiple proteases. The quantified peptides of these plasma proteins spanned 3 orders of magnitude (0.02-70 μM). In total, 60 peptides were used for absolute quantification and the majority of the peptides showed high robustness. The retained reproducibility was achieved by quantifying plasma proteins using spiked stable isotope standard recombinant proteins in a targeted proteomics workflow. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a absolute quantification | |
653 | a multiple proteases | |
653 | a plasma proteomics | |
653 | a SRM | |
653 | a targeted proteomics | |
653 | a Blood Proteins | |
653 | a Endopeptidases | |
653 | a Humans | |
653 | a Isotope Labeling | |
653 | a Isotopes | |
653 | a Peptide Hydrolases | |
653 | a Peptides | |
653 | a Proteomics | |
653 | a Recombinant Proteins | |
653 | a Reproducibility of Results | |
653 | a peptide | |
653 | a plasma protein | |
653 | a proteinase | |
653 | a recombinant protein | |
653 | a stable isotope | |
653 | a isotope | |
653 | a peptide hydrolase | |
653 | a Article | |
653 | a controlled study | |
653 | a female | |
653 | a human | |
653 | a kernel method | |
653 | a male | |
653 | a mass spectrometry | |
653 | a plasma | |
653 | a quantitative analysis | |
653 | a reproducibility | |
653 | a procedures | |
700 | 1 | a Kotol, Davidu KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap4 aut0 (Swepub:kth)u1yd1hdh |
700 | 1 | a Hober, Andreas,d 1992-u KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u15bkxsl |
700 | 1 | a Uhlén, Mathiasu KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap4 aut0 (Swepub:kth)u1dulvmw |
700 | 1 | a Edfors, Fredriku KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u149ml0e |
710 | 2 | a KTHb Science for Life Laboratory, SciLifeLab4 org |
773 | 0 | t Journal of Proteome Researchd : American Chemical Society (ACS)g 21:10, s. 2526-2534q 21:10<2526-2534x 1535-3893x 1535-3907 |
856 | 4 | u https://doi.org/10.1021/acs.jproteome.2c00491y Fulltext |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-327264 |
856 | 4 8 | u https://doi.org/10.1021/acs.jproteome.2c00491 |
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