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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004549naa a2200385 4500
001oai:DiVA.org:his-1672
003SwePub
008071001s2005 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-16722 URI
0247 a https://doi.org/10.1093/jxb/eri2422 DOI
040 a (SwePub)his
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Stangeland, Biljanau Plant Molecular Biology Laboratory, Department of Plant and Environmental Sciences, University of Life Sciences, PO Box 5003, N-1432 Ås Norway, Norway / National Hospital, Section for Gene Therapy, N-0027 Oslo, Norway4 aut
2451 0a Molecular analysis of Arabidopsis endosperm and embryo promoter trap lines :b Reporter-gene expression can result from T-DNA insertions in antisense orientation, in introns and in intergenic regions, in addition to sense insertion at the 5′ end of genes
264 c 2005-07-12
264 1b Oxford University Press,c 2005
338 a print2 rdacarrier
520 a Random insertions of promoterless reporter genes in genomes are a common tool for identifying marker lines with tissue-specific expression patterns. Such lines are assumed to reflect the activity of endogenous promoters and should facilitate the cloning of genes expressed in the corresponding tissues. To identify genes active in seed organs, plant DNA flanking T-DNA insertions (T-DNAs) have been cloned in 16 Arabidopsis thaliana GUS-reporter lines. T-DNAs were found in proximal promoter regions, 5' UTR or intron with GUS in the same (sense) orientation as the tagged gene, but contrary to expectations also in inverted orientation in the 5' end of genes or in intergenic regions. RT-PCR, northern analysis, and data on expression patterns of tagged genes, compared with the expression pattern of the reporter lines, suggest that the expression pattern of a reporter gene will reflect the pattern of a tagged gene when inserted in sense orientation in the 5' UTR or intron. When inserted in the promoter region, the reporter-gene expression patterns may be restricted compared with the endogenous gene. Among the trapped genes, the previously described nitrate transporter gene AtNRT1.1, the cyclophilin gene ROC3, and the histone deacetylase gene AtHD2C were found. Reporter-gene expression when positioned in antisense orientation, for example, in the SLEEPY1 gene, is indicative of antisense expression of the tagged gene. For T-DNAs found in intergenic regions, it is suggested that the reporter gene is transcribed from cryptic promoters or promoters of as yet unannotated genes.
653 a Naturvetenskap
653 a Natural sciences
7001 a Nestestog, Ragnhildu Plant Molecular Biology Laboratory, Department of Plant and Environmental Sciences, University of Life Sciences, PO Box 5003, N-1432 Ås Norway, Norway4 aut
7001 a Grini, Paul E.u Department of Molecular Biosciences, University of Oslo, Oslo, PO Box 1041 Blindern, N-0316 Oslo, Norway4 aut
7001 a Skrbo, Nirmau Department of Molecular Biosciences, University of Oslo, Oslo, PO Box 1041 Blindern, N-0316 Oslo, Norway4 aut
7001 a Berg, Anitau Department of Molecular Biosciences, University of Oslo, Oslo, PO Box 1041 Blindern, N-0316 Oslo, Norway4 aut
7001 a Salehian, Zhianu Plant Molecular Biology Laboratory, Department of Plant and Environmental Sciences, University of Life Sciences, PO Box 5003, N-1432 Ås Norway, Norway4 aut
7001 a Mandal, Abulu Högskolan i Skövde,Institutionen för kommunikation och information4 aut0 (Swepub:his)mana
7001 a Aalen, Reidunn B.u Department of Molecular Biosciences, University of Oslo, Oslo, PO Box 1041 Blindern, N-0316 Oslo, Norway4 aut
7102 a Plant Molecular Biology Laboratory, Department of Plant and Environmental Sciences, University of Life Sciences, PO Box 5003, N-1432 Ås Norway, Norway / National Hospital, Section for Gene Therapy, N-0027 Oslo, Norwayb Plant Molecular Biology Laboratory, Department of Plant and Environmental Sciences, University of Life Sciences, PO Box 5003, N-1432 Ås Norway, Norway4 org
7730 t Journal of Experimental Botanyd : Oxford University Pressg 56:419, s. 2495-2505q 56:419<2495-2505x 0022-0957x 1460-2431
8564 u https://academic.oup.com/jxb/article-pdf/56/419/2495/1421941/eri242.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-1672
8564 8u https://doi.org/10.1093/jxb/eri242

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