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Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetrating peptide
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- Dowaidar, Moataz (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Abdelhamid, Hani Nasser (författare)
- Stockholms universitet,Institutionen för material- och miljökemi (MMK),Assuit University, Egypt
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- Hällbrink, Mattias (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- 2018-09-17
- 2018
- Engelska.
- Ingår i: Journal of biomaterials applications. - : SAGE Publications. - 0885-3282 .- 1530-8022. ; 33:3, s. 392-401
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Gene-based therapies, including the delivery of oligonucleotides, offer promising methods for the treatment of cancer cells. However, they have various limitations including low efficiency. Herein, cell-penetrating peptides (CPPs)-conjugated chitosan-modified iron oxide magnetic nanoparticles (CPPs-CTS@MNPs) with high biocompatibility as well as high efficiency were tested for the delivery of oligonucleotides such as plasmid pGL3, splice correction oligonucleotides, and small-interfering RNA. A biocompatible nanocomposite, in which CTS@MNPs was incorporated in non-covalent complex with CPPs-oligonucleotide, is developed. Modifying the surface of magnetic nanoparticles with cationic chitosan-modified iron oxide improved the performance of magnetic nanoparticles-CPPs for oligonucleotide delivery. CPPs-CTS@MNPs complexes enhance oligonucleotide transfection compared to CPPs@MNPs or CPPs. The hydrophilic character of CTS@MNPs improves complexation with plasmid pGL3, splice correction oligonucleotides, and small-interfering RNA payload, which consequently resulted in not only strengthening the colloidal stability of the constructed complex but also improving their biocompatibility. Transfection using PF14-splice correction oligonucleotides-CTS@MNPs showed sixfold increase of the transfection compared to splice correction oligonucleotides-PF14 that showed higher transfection than the commercially available lipid-based vector Lipofectamine™ 2000. Nanoscaled CPPs-CTS@MNPs comprise a new family of biomaterials that can circumvent some of the limitations of CPPs or magnetic nanoparticles.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Cell-penetrating peptides
- magnetic nanoparticles
- chitosan
- gene delivery
- small-interfering RNA
- splice correction oligonucleotides
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- Langel, Ülo
- Zou, Xiaodong
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