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In vivo protein crystallization opens new routes in structural biology

Koopmann, Rudolf (author)
Cupelli, Karolina (author)
Redecke, Lars (author)
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Nass, Karol (author)
DePonte, Daniel P (author)
White, Thomas A (author)
Stellato, Francesco (author)
Rehders, Dirk (author)
Liang, Mengning (author)
Andreasson, Jakob (author)
Uppsala universitet,Molekylär biofysik
Aquila, Andrew (author)
Bajt, Sasa (author)
Barthelmess, Miriam (author)
Barty, Anton (author)
Bogan, Michael J (author)
Bostedt, Christoph (author)
Boutet, Sebastien (author)
Bozek, John D (author)
Caleman, Carl (author)
Coppola, Nicola (author)
Davidsson, Jan (author)
Uppsala universitet,Fysikalisk kemi
Doak, R Bruce (author)
Ekeberg, Tomas (author)
Uppsala universitet,Molekylär biofysik
Epp, Sascha W (author)
Erk, Benjamin (author)
Fleckenstein, Holger (author)
Foucar, Lutz (author)
Graafsma, Heinz (author)
Gumprecht, Lars (author)
Hajdu, Janos (author)
Uppsala universitet,Molekylär biofysik
Hampton, Christina Y (author)
Hartmann, Andreas (author)
Hartmann, Robert (author)
Hauser, Gunter (author)
Hirsemann, Helmut (author)
Holl, Peter (author)
Hunter, Mark S (author)
Kassemeyer, Stephan (author)
Kirian, Richard A (author)
Lomb, Lukas (author)
Maia, Filipe R N C (author)
Kimmel, Nils (author)
Martin, Andrew V (author)
Messerschmidt, Marc (author)
Reich, Christian (author)
Rolles, Daniel (author)
Rudek, Benedikt (author)
Rudenko, Artem (author)
Schlichting, Ilme (author)
Schulz, Joachim (author)
Seibert, M Marvin (author)
Uppsala universitet,Molekylär biofysik
Shoeman, Robert L (author)
Sierra, Raymond G (author)
Soltau, Heike (author)
Stern, Stephan (author)
Struder, Lothar (author)
Timneanu, Nicusor (author)
Uppsala universitet,Molekylär biofysik
Ullrich, Joachim (author)
Wang, Xiaoyu (author)
Weidenspointner, Georg (author)
Weierstall, Uwe (author)
Williams, Garth J (author)
Wunderer, Cornelia B (author)
Fromme, Petra (author)
Spence, John C H (author)
Stehle, Thilo (author)
Chapman, Henry N (author)
Betzel, Christian (author)
Duszenko, Michael (author)
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 (creator_code:org_t)
2012-01-29
2012
English.
In: Nature Methods. - : Springer Science and Business Media LLC. - 1548-7091 .- 1548-7105. ; 9:3, s. 259-262
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Subject headings

NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)

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