WFRF:(Burke Terry)
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LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02583naa a2200361 4500 | |
| 001 | oai:DiVA.org:uu-206868 | |
| 003 | SwePub | |
| 008 | 130905s2012 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2068682 URI |
| 024 | 7 | a https://doi.org/10.1155/2012/2816932 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Ekblom, Robertu Uppsala universitet,Evolutionsbiologi4 aut0 (Swepub:uu)roekb519 |
| 245 | 1 0 | a Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies |
| 264 | 1 | b Hindawi Limited,c 2012 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Next-generation sequencing of transcriptomes (RNA-Seq) is being used increasingly in studies of nonmodel organisms. Here, we evaluate the effectiveness of normalising cDNA libraries prior to sequencing in a small-scale study of the zebra finch. We find that assemblies produced from normalised libraries had a larger number of contigs but used fewer reads compared to unnormalised libraries. Considerably more genes were also detected using the contigs produced from normalised cDNA, and microsatellite discovery was up to 73% more efficient in these. There was a positive correlation between the detected expression level of genes in normalised and unnormalised cDNA, and there was no difference in the number of genes identified as being differentially expressed between blood and spleen for the normalised and unnormalised libraries. We conclude that normalised cDNA libraries are preferable for many applications of RNA-Seq and that these can also be used in quantitative gene expression studies. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Genetik0 (SwePub)106092 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Genetics0 (SwePub)106092 hsv//eng |
| 700 | 1 | a Slate, Jon4 aut |
| 700 | 1 | a Horsburgh, Gavin J4 aut |
| 700 | 1 | a Birkhead, Tom4 aut |
| 700 | 1 | a Burke, Terry4 aut |
| 710 | 2 | a Uppsala universitetb Evolutionsbiologi4 org |
| 773 | 0 | t Comparative and functional genomicsd : Hindawi Limitedg 2012, s. 281693-q 2012<281693-x 1531-6912x 1532-6268 |
| 856 | 4 | u https://doi.org/10.1155/2012/281693y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:645780/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://doi.org/10.1155/2012/281693 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-206868 |
| 856 | 4 8 | u https://doi.org/10.1155/2012/281693 |
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