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A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR.
- White, H (author)
- Deprez, L (author)
- Corbisier, P (author)
- show more...
- Hall, V (author)
- Lin, F (author)
- Mazoua, S (author)
- Trapmann, S (author)
- Aggerholm, A (author)
- Andrikovics, H (author)
- Akiki, S (author)
- Boeckx, N (author)
- Bench, A (author)
- Catherwood, M (author)
- Chudleigh, S (author)
- Clench, T (author)
- Colomer, D (author)
- Daraio, F (author)
- Dulucq, S (author)
- Farrugia, J (author)
- Fletcher, L (author)
- Foroni, L (author)
- Ganderton, R (author)
- Gerrard, G (author)
- Gineikienė, E (author)
- Hayette, S (author)
- El Housni, H (author)
- Izzo, B (author)
- Jansson, M (author)
- Jurcek, T (author)
- Kairisto, V (author)
- Kizilors, A (author)
- Kim, D-W (author)
- Lange, T (author)
- Lion, T (author)
- Polakova, K M (author)
- Martinelli, G (author)
- McCarron, S (author)
- Merle, P A (author)
- Milner, B (author)
- Mitterbauer-Hohendanner, G (author)
- Nagar, M (author)
- Nickless, G (author)
- Nomdedéu, J (author)
- Nymoen, D A (author)
- Leibundgut, E O (author)
- Ozbek, U (author)
- Pajič, T (author)
- Pfeifer, H (author)
- Preudhomme, C (author)
- Raudsepp, K (author)
- Romeo, G (author)
- Sacha, T (author)
- Talmaci, R (author)
- Touloumenidou, T (author)
- Van der Velden, V H J (author)
- Waits, P (author)
- Wang, L (author)
- Wilkinson, E (author)
- Wilson, G (author)
- Wren, D (author)
- Zadro, R (author)
- Ziermann, J (author)
- Zoi, K (author)
- Müller, M C (author)
- Hochhaus, A (author)
- Schimmel, H (author)
- Cross, N C P (author)
- Emons, H (author)
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- (creator_code:org_t)
- 2014-07-18
- 2015
- English.
- In: Leukemia. - : Springer Science and Business Media LLC. - 1476-5551 .- 0887-6924. ; 29:2, s. 369-376
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukemia, but there is substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 10(6), 1.08±0.11 × 10(5), 1.03±0.10 × 10(4), 1.02±0.09 × 10(3), 1.04±0.10 × 10(2) and 10.0±1.5 copies/μL. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR-ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise the numbers of measured transcripts of e14a2 BCR-ABL1 and three control genes; ABL1, BCR and GUSB. The set of 6 plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (http://irmm.jrc.ec.europa.eu; CRM code ERM-AD623a-f).Leukemia accepted article preview online, 18 July 2014; doi:10.1038/leu.2014.217.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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Find more in SwePub
- By the author/editor
- White, H
- Deprez, L
- Corbisier, P
- Hall, V
- Lin, F
- Mazoua, S
- show more...
- Trapmann, S
- Aggerholm, A
- Andrikovics, H
- Akiki, S
- Barbany, G
- Boeckx, N
- Bench, A
- Catherwood, M
- Jansson, Mattias
- Chudleigh, S
- Clench, T
- Colomer, D
- Daraio, F
- Dulucq, S
- Farrugia, J
- Fletcher, L
- Foroni, L
- Ganderton, R
- Gerrard, G
- Gineikienė, E
- Hayette, S
- El Housni, H
- Izzo, B
- Jansson, M
- Johnels, Petra
- Jurcek, T
- Kairisto, V
- Kizilors, A
- Kim, D-W
- Lange, T
- Lion, T
- Polakova, K M
- Martinelli, G
- McCarron, S
- Merle, P A
- Milner, B
- Mitterbauer-Hohe ...
- Nagar, M
- Nickless, G
- Nomdedéu, J
- Nymoen, D A
- Leibundgut, E O
- Ozbek, U
- Pajič, T
- Pfeifer, H
- Preudhomme, C
- Raudsepp, K
- Romeo, G
- Sacha, T
- Talmaci, R
- Touloumenidou, T
- Van der Velden, ...
- Waits, P
- Wang, L
- Wilkinson, E
- Wilson, G
- Wren, D
- Zadro, R
- Ziermann, J
- Zoi, K
- Müller, M C
- Hochhaus, A
- Schimmel, H
- Cross, N C P
- Emons, H
- show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Medical Genetics
- Articles in the publication
- Leukemia
- By the university
- Lund University
- Uppsala University
- Karolinska Institutet
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