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WASP and SCAR have ...
WASP and SCAR have distinct roles in activating the Arp2/3 complex during myoblast fusion
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Berger, Susanne (author)
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Schäfer, Gritt (author)
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Kesper, Dörthe A (author)
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Holz, Anne (author)
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- Eriksson, Therese (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Palmer
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- Palmer, Ruth H (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Palmer
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Beck, Lothar (author)
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Klämbt, Christian (author)
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Renkawitz-Pohl, Renate (author)
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Onel, Susanne-Filiz (author)
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(creator_code:org_t)
- The Company of Biologists, 2008
- 2008
- English.
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In: Journal of Cell Science. - : The Company of Biologists. - 0021-9533 .- 1477-9137. ; 121:Pt 8, s. 1303-1313
- Related links:
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http://jcs.biologist...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Myoblast fusion takes place in two steps in mammals and in Drosophila. First, founder cells (FCs) and fusion-competent myoblasts (FCMs) fuse to form a trinucleated precursor, which then recruits further FCMs. This process depends on the formation of the fusion-restricted myogenic-adhesive structure (FuRMAS), which contains filamentous actin (F-actin) plugs at the sites of cell contact. Fusion relies on the HEM2 (NAP1) homolog Kette, as well as Blow and WASP, a member of the Wiskott-Aldrich-syndrome protein family. Here, we show the identification and characterization of schwächling--a new Arp3-null allele. Ultrastructural analyses demonstrate that Arp3 schwächling mutants can form a fusion pore, but fail to integrate the fusing FCM. Double-mutant experiments revealed that fusion is blocked completely in Arp3 and wasp double mutants, suggesting the involvement of a further F-actin regulator. Indeed, double-mutant analyses with scar/WAVE and with the WASP-interacting partner vrp1 (sltr, wip)/WIP show that the F-actin regulator scar also controls F-actin formation during myoblast fusion. Furthermore, the synergistic phenotype observed in Arp3 wasp and in scar vrp1 double mutants suggests that WASP and SCAR have distinct roles in controlling F-actin formation. From these findings we derived a new model for actin regulation during myoblast fusion.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Drosophila
- Myogenesis
- F-actin
- Arp3
- FuRMAS
- Actin cytoskeleton
- kette
- Molecular biology
- Molekylärbiologi
- molekylärbiologi
- Molecular Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Berger, Susanne
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Schäfer, Gritt
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Kesper, Dörthe A
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Holz, Anne
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Eriksson, Theres ...
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Palmer, Ruth H
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show more...
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Beck, Lothar
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Klämbt, Christia ...
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Renkawitz-Pohl, ...
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Onel, Susanne-Fi ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Journal of Cell ...
- By the university
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Umeå University