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The catalytic acid-...
The catalytic acid-base in GH109 resides in a conserved GGHGG loop and allows for comparable α-retaining and β-inverting activity in an N-acetylgalactosaminidase from Akkermansia muciniphila
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- Teze, David (författare)
- Technical University of Denmark
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- Shuoker, Bashar (författare)
- Lund University
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- Chaberski, Evan Kirk (författare)
- Novo Nordisk Foundation
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- Kunstmann, Sonja (författare)
- Technical University of Denmark
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- Fredslund, Folmer (författare)
- Novo Nordisk Foundation
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- Nordberg Karlsson, Eva (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Welner, Ditte Hededam (författare)
- Novo Nordisk Foundation
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- Abou Hachem, Maher (författare)
- Technical University of Denmark
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(creator_code:org_t)
- American Chemical Society (ACS), 2019
- Engelska.
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Ingår i: ChemRxiv. - : American Chemical Society (ACS).
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://backend.orbi...
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https://lup.lub.lu.s...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- The study describes the first glycoside hydrolase that exhibits comparable levels of activity on α- and β-linked saccharide substrates. This enzyme, assigned into GH109, is encoded by the genome of the human gut symbiont Akkermansia muciniphila that is a model primary degrader of the heavily O-glycosylated mucin glycoprotein that coats the epithelial enterocytes.The elusive catalytic acid/base catalyst in GH109 enzymes is identified as a histidine that is presented by a flexible loop that positions it for catalysis on both α- and β-substrates. This dual activity may be an evolutionary adaptation to extend the range of substrates targeted by a single non-canonical NAD+-dependant GH.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
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