WFRF:(Chubinskaya Susan)
Sökning: WFRF:(Chubinskaya Susan) > (2017) > Quantitative proteo...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04138naa a2200409 4500 | |
| 001 | oai:lup.lub.lu.se:fb3fc69e-5c9b-4dd8-8ccf-6163c3400dfd | |
| 003 | SwePub | |
| 008 | 170127s2017 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/fb3fc69e-5c9b-4dd8-8ccf-6163c3400dfd2 URI |
| 024 | 7 | a https://doi.org/10.1016/j.matbio.2016.12.0042 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Wang, Yangu Massachusetts Institute of Technology4 aut |
| 245 | 1 0 | a Quantitative proteomics analysis of cartilage response to mechanical injury and cytokine treatment |
| 264 | 1 | b Elsevier BV,c 2017 |
| 520 | a Mechanical damage at the time of joint injury and the ensuing inflammatory response associated with elevated levels of pro-inflammatory cytokines in the synovial fluid, are reported to contribute to the progression to osteoarthritis after injury. In this exploratory study, we used a targeted proteomics approach to follow the progression of matrix degradation in response to mechanical damage and cytokine treatment of human knee cartilage explants, and thereby to study potential molecular biomarkers. This proteomics approach allowed us to unambiguously identify and quantify multiple peptides and proteins in the cartilage medium and explants upon treatment with ±. injurious compression ±. cytokines, treatments that mimic the earliest events in post-traumatic OA. We followed degradation of different protein domains, e.g., G1/G2/G3 of aggrecan, by measuring representative peptides of matrix proteins released into the medium at 7 time points throughout the 21-day culture period. COMP neo-epitopes, which were previously identified in the synovial fluid of knee injury/OA patients, were also released by these human cartilage explants treated with cyt and cyt+inj. The absence of collagen pro-peptides and elevated levels of specific COMP and COL3A1 neo-epitopes after human knee trauma may be relevant as potential biomarkers for post-traumatic OA. This model system thereby enables study of the kinetics of cartilage degradation and the identification of biomarkers within cartilage explants and those released to culture medium. Discovery proteomics revealed that candidate proteases were identified after specific treatment conditions, including MMP1, MMP-3, MMP-10 and MMP-13. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng |
| 653 | a Cartilage matrix | |
| 653 | a Cytokines | |
| 653 | a Mass spectrometry | |
| 653 | a Post-traumatic osteoarthritis | |
| 653 | a Proteomics | |
| 700 | 1 | a Li, Yangu Massachusetts Institute of Technology4 aut |
| 700 | 1 | a Khabut, Areeju Lund University,Lunds universitet,Reumatologi och molekylär skelettbiologi,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Rheumatology,Section III,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)med-akt |
| 700 | 1 | a Chubinskaya, Susanu Rush University4 aut |
| 700 | 1 | a Grodzinsky, Alan J.u Massachusetts Institute of Technology4 aut0 (Swepub:lu)med-agz |
| 700 | 1 | a Önnerfjord, Patriku Lund University,Lunds universitet,Reumatologi och molekylär skelettbiologi,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Rheumatology,Section III,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)akem-pon |
| 710 | 2 | a Massachusetts Institute of Technologyb Reumatologi och molekylär skelettbiologi4 org |
| 773 | 0 | t Matrix Biologyd : Elsevier BVg 63, s. 11-22q 63<11-22x 0945-053X |
| 856 | 4 | u http://dx.doi.org/10.1016/j.matbio.2016.12.004y FULLTEXT |
| 856 | 4 | u https://europepmc.org/articles/pmc5472506?pdf=render |
| 856 | 4 8 | u https://lup.lub.lu.se/record/fb3fc69e-5c9b-4dd8-8ccf-6163c3400dfd |
| 856 | 4 8 | u https://doi.org/10.1016/j.matbio.2016.12.004 |
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