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Sökning: WFRF:(Degn Matilda) > (2016) > Conditional ablatio...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004452naa a2200505 4500
001oai:lup.lub.lu.se:faf81412-47bc-4956-83c0-8645bd716430
003SwePub
008170112s2016 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/faf81412-47bc-4956-83c0-8645bd7164302 URI
0247 a https://doi.org/10.1038/srep292912 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Clausen, Bettina Hjelmu University of Southern Denmark4 aut
2451 0a Conditional ablation of myeloid TNF increases lesion volume after experimental stroke in mice, possibly via altered ERK1/2 signaling
264 c 2016-07-07
264 1b Springer Science and Business Media LLC,c 2016
520 a Microglia are activated following cerebral ischemia and increase their production of the neuro- and immunomodulatory cytokine tumor necrosis factor (TNF). To address the function of TNF from this cellular source in focal cerebral ischemia we used TNF conditional knock out mice (LysMcreTNF fl/fl) in which the TNF gene was deleted in cells of the myeloid lineage, including microglia. The deletion reduced secreted TNF levels in lipopolysaccharide-stimulated cultured primary microglia by ∼93%. Furthermore, phosphorylated-ERK/ERK ratios were significantly decreased in naïve LysMcreTNF fl/fl mice demonstrating altered ERK signal transduction. Micro-PET using 18 [F]-fluorodeoxyglucose immediately after focal cerebral ischemia showed increased glucose uptake in LysMcreTNF fl/fl mice, representing significant metabolic changes, that translated into increased infarct volumes at 24 hours and 5 days compared to littermates (TNFfl/fl). In naïve LysMcreTNF fl/fl mice cytokine levels were low and comparable to littermates. At 6 hours, TNF producing microglia were reduced by 56% in the ischemic cortex in LysMcreTNF fl/fl mice compared to littermate mice, whereas no TNF + leukocytes were detected. At 24 hours, pro-inflammatory cytokine (TNF, IL-1β, IL-6, IL-5 and CXCL1) levels were significantly lower in LysMcreTNF fl/fl mice, despite comparable infiltrating leukocyte populations. Our results identify microglial TNF as beneficial and neuroprotective in the acute phase and as a modulator of neuroinflammation at later time points after experimental ischemia, which may contribute to regenerative recovery.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Neurologi0 (SwePub)302072 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Neurology0 (SwePub)302072 hsv//eng
7001 a Degn, Matildau Copenhagen University Hospital4 aut
7001 a Sivasaravanaparan, Mithulau University of Southern Denmark4 aut
7001 a Fogtmann, Torbenu University of Southern Denmark4 aut
7001 a Andersen, Maria Gammelstrupu University of Southern Denmark4 aut
7001 a Trojanowsky, Michelle D.u University of Miami4 aut
7001 a Gao, Hanu University of Miami4 aut
7001 a Hvidsten, Svendu Odense University Hospital4 aut
7001 a Baun, Christinau Odense University Hospital4 aut
7001 a Deierborg, Tomasu Lund University,Lunds universitet,Neuroinflammation,Forskargrupper vid Lunds universitet,Lund University Research Groups4 aut0 (Swepub:lu)expb-tol
7001 a Finsen, Benteu University of Southern Denmark4 aut
7001 a Kristensen, Bjarne Wintheru Odense University Hospital,University of Southern Denmark4 aut
7001 a Bak, Sara Thornbyu University of Southern Denmark4 aut
7001 a Meyer, Mortenu University of Southern Denmark4 aut
7001 a Lee, Jaeu University of Miami4 aut
7001 a Nedospasov, Sergei A.u P.N. Lebedev Physical Institute of the Russian Academy of Sciences,Lomonosov Moscow State University4 aut
7001 a Brambilla, Robertau University of Miami4 aut
7001 a Lambertsen, Kate Lykkeu University of Southern Denmark,Odense University Hospital4 aut
7102 a University of Southern Denmarkb Copenhagen University Hospital4 org
7730 t Scientific Reportsd : Springer Science and Business Media LLCg 6q 6x 2045-2322
8564 u http://dx.doi.org/10.1038/srep29291x freey FULLTEXT
8564 u https://www.nature.com/articles/srep29291.pdf
8564 8u https://lup.lub.lu.se/record/faf81412-47bc-4956-83c0-8645bd716430
8564 8u https://doi.org/10.1038/srep29291

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