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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004764naa a2200433 4500
001oai:DiVA.org:mau-5643
003SwePub
008200228s2018 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-56432 URI
0247 a https://doi.org/10.1038/s41598-018-24154-z2 DOI
040 a (SwePub)mau
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Hedegaard, Sofie Foghu Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark4 aut
2451 0a Fluorophore labeling of a cell-penetrating peptide significantly alters the mode and degree of biomembrane interaction
264 c 2018-04-20
264 1b Nature Publishing Group,c 2018
338 a electronic2 rdacarrier
520 a The demand for highly efficient macromolecular drugs, used in the treatment of many severe diseases, is continuously increasing. However, the hydrophilic character and large molecular size of these drugs significantly limit their ability to permeate across cellular membranes and thus impede the drugs in reaching their target sites in the body. Cell-penetrating peptides (CPP) have gained attention as promising drug excipients, since they can facilitate drug permeation across cell membranes constituting a major biological barrier. Fluorophores are frequently covalently conjugated to CPPs to improve detection, however, the ensuing change in physico-chemical properties of the CPPs may alter their biological properties. With complementary biophysical techniques, we show that the mode of biomembrane interaction may change considerably upon labeling of the CPP penetratin (PEN) with a fluorophore. Fluorophore-PEN conjugates display altered modes of membrane interaction with increased insertion into the core of model cell membranes thereby exerting membrane-thinning effects. This is in contrast to PEN, which localizes along the head groups of the lipid bilayer, without affecting the thickness of the lipid tails. Particularly high membrane disturbance is observed for the two most hydrophobic PEN conjugates; rhodamine B or 1-pyrene butyric acid, as compared to the four other tested fluorophore-PEN conjugates.
653 a Multidisciplinary Sciences
7001 a Derbas, Mohammed Sobhiu Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark4 aut
7001 a Lind, Tania Kjellerupu Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV)4 aut0 (Swepub:mau)ag5616
7001 a Kasimova, Marina Robertnovau Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark; Symphogen A/S, Pederstrupvej 93, 2750, Ballerup, Denmark4 aut
7001 a Christensen, Malene Vintheru Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 162, 2100, Copenhagen, Denmark4 aut
7001 a Michaelsen, Maria Hotoftu Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark4 aut
7001 a Campbell, Richard A.u Institut Laue-Langevin, 71 avenue des Martyrs, CS20156, 38042, Grenoble, France4 aut
7001 a Jorgensen, Leneu Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark4 aut
7001 a Franzyk, Henriku Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 162, 2100, Copenhagen, Denmark4 aut
7001 a Cárdenas, Maritéu Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV)4 aut0 (Swepub:mau)ae0614
7001 a Nielsen, Hanne Morcku Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark4 aut
7102 a Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmarkb Institutionen för biomedicinsk vetenskap (BMV)4 org
7730 t Scientific Reportsd : Nature Publishing Groupg 8:1q 8:1x 2045-2322
8564 u https://doi.org/10.1038/s41598-018-24154-zy Fulltext
8564 u https://mau.diva-portal.org/smash/get/diva2:1402507/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://www.nature.com/articles/s41598-018-24154-z.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-5643
8564 8u https://doi.org/10.1038/s41598-018-24154-z

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