Sökning: WFRF:(Fuchs Jeanette) > (2008) > Multiplexed Lipid D...
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000 | 03829naa a2200493 4500 | |
001 | oai:lup.lub.lu.se:5c0abfc2-e813-44c0-8449-75edc635d82b | |
003 | SwePub | |
008 | 160401s2008 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/12840972 URI |
024 | 7 | a https://doi.org/10.1002/smll.2008009492 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Sekula, Sylwia4 aut |
245 | 1 0 | a Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture |
264 | 1 | b Wiley,c 2008 |
520 | a Molecular patterning processes taking place in biological systems are challenging to study in vivo because of their dynamic behavior, subcellular size, and high degree of complexity. In vitro patterning of biomolecules using nanolithography allows simplification of the processes and detailed study of the dynamic interactions. Parallel dip-pen nanolithography (DPN) is uniquely capable of integrating functional biomolecules on subcellular length scales due to its constructive nature, high resolution, and high throughput. Phospholipids are particularly well suited as inks for DPN since a variety of different functional lipids can be readily patterned in parallel. Here DPN is used to spatially pattern multicomponent micro- and nano-structured supported lipid membranes and multilayers that are fluid and contain various amounts of biotin and/or nitrilotriacetic acid functional groups. The patterns are characterized by fluorescence microscopy and photoemission electron microscopy. Selective adsorption of functionalized or recombinant proteins based on streptavidin or histidine-tag coupling enables the semisynthetic fabrication of model peripheral membrane bound proteins. The biomimetic membrane patterns formed in this way are then used as substrates for cell culture, as demonstrated by the selective adhesion and activation of T-cells. | |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Nanoteknikx Nanoteknik0 (SwePub)210012 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Nano-technologyx Nano-technology0 (SwePub)210012 hsv//eng |
653 | a proteomics | |
653 | a phospholipids | |
653 | a dip-pen nanolithography | |
653 | a biomimetics | |
653 | a cell adhesion | |
700 | 1 | a Fuchs, Jeanette4 aut |
700 | 1 | a Weg-Remers, Susanne4 aut |
700 | 1 | a Nagel, Peter4 aut |
700 | 1 | a Schuppler, Stefan4 aut |
700 | 1 | a Fragala, Joe4 aut |
700 | 1 | a Theilacker, Nora4 aut |
700 | 1 | a Franueb, Matthias4 aut |
700 | 1 | a Wingren, Christeru Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)immt-cwi |
700 | 1 | a Ellmark, Peteru Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)immt-pel |
700 | 1 | a Borrebaeck, Carlu Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)immt-cbo |
700 | 1 | a Mirkin, Chad A.4 aut |
700 | 1 | a Fuchs, Harald4 aut |
700 | 1 | a Lenhert, Steven4 aut |
710 | 2 | a Institutionen för immunteknologib Institutioner vid LTH4 org |
773 | 0 | t Smalld : Wileyg 4:10, s. 1785-1793q 4:10<1785-1793x 1613-6829x 1613-6810 |
856 | 4 | u http://dx.doi.org/10.1002/smll.200800949y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/1284097 |
856 | 4 8 | u https://doi.org/10.1002/smll.200800949 |
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