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Sökning: WFRF:(Fuchs Jeanette) > (2008) > Multiplexed Lipid D...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003829naa a2200493 4500
001oai:lup.lub.lu.se:5c0abfc2-e813-44c0-8449-75edc635d82b
003SwePub
008160401s2008 | |||||||||||000 ||eng|
024a https://lup.lub.lu.se/record/12840972 URI
024a https://doi.org/10.1002/smll.2008009492 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
100a Sekula, Sylwia4 aut
2451 0a Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture
264 1b Wiley,c 2008
520 a Molecular patterning processes taking place in biological systems are challenging to study in vivo because of their dynamic behavior, subcellular size, and high degree of complexity. In vitro patterning of biomolecules using nanolithography allows simplification of the processes and detailed study of the dynamic interactions. Parallel dip-pen nanolithography (DPN) is uniquely capable of integrating functional biomolecules on subcellular length scales due to its constructive nature, high resolution, and high throughput. Phospholipids are particularly well suited as inks for DPN since a variety of different functional lipids can be readily patterned in parallel. Here DPN is used to spatially pattern multicomponent micro- and nano-structured supported lipid membranes and multilayers that are fluid and contain various amounts of biotin and/or nitrilotriacetic acid functional groups. The patterns are characterized by fluorescence microscopy and photoemission electron microscopy. Selective adsorption of functionalized or recombinant proteins based on streptavidin or histidine-tag coupling enables the semisynthetic fabrication of model peripheral membrane bound proteins. The biomimetic membrane patterns formed in this way are then used as substrates for cell culture, as demonstrated by the selective adhesion and activation of T-cells.
650 7a TEKNIK OCH TEKNOLOGIERx Nanoteknikx Nanoteknik0 (SwePub)210012 hsv//swe
650 7a ENGINEERING AND TECHNOLOGYx Nano-technologyx Nano-technology0 (SwePub)210012 hsv//eng
653 a proteomics
653 a phospholipids
653 a dip-pen nanolithography
653 a biomimetics
653 a cell adhesion
700a Fuchs, Jeanette4 aut
700a Weg-Remers, Susanne4 aut
700a Nagel, Peter4 aut
700a Schuppler, Stefan4 aut
700a Fragala, Joe4 aut
700a Theilacker, Nora4 aut
700a Franueb, Matthias4 aut
700a Wingren, Christeru Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)immt-cwi
700a Ellmark, Peteru Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)immt-pel
700a Borrebaeck, Carlu Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)immt-cbo
700a Mirkin, Chad A.4 aut
700a Fuchs, Harald4 aut
700a Lenhert, Steven4 aut
710a Institutionen för immunteknologib Institutioner vid LTH4 org
773t Smalld : Wileyg 4:10, s. 1785-1793q 4:10<1785-1793x 1613-6829x 1613-6810
856u http://dx.doi.org/10.1002/smll.200800949y FULLTEXT
8564 8u https://lup.lub.lu.se/record/1284097
8564 8u https://doi.org/10.1002/smll.200800949

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