Sökning: WFRF:(Gigolashvili T.)
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HAG2/MYB76 and HAG3...
HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in Arabidopsis thaliana
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Gigolashvili, T. (författare)
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- Engqvist, Martin, 1983 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Yatusevich, R. (författare)
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Müller, C. (författare)
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Flügge, U.I. (författare)
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(creator_code:org_t)
- 2007-11-28
- 2008
- Engelska.
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Ingår i: New Phytologist. - : Wiley. - 1469-8137 .- 0028-646X. ; 177:3, s. 627-642
- Relaterad länk:
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https://nph.onlineli...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In a previous transactivation screen, two Arabidopsis thaliana R2R3-MYB transcription factors, HAG2/MYB76 and HAG3/MYB29, along with the already characterized HAG1/MYB28, were identified as putative regulators of aliphatic glucosinolate biosynthesis. • Molecular and biochemical characterization of HAG2/MYB76 and HAG3/MYB29 functions was performed using transformants with increased or repressed transcript levels. Real-time PCR assays, cotransformation assays and measurements of glucosinolate contents were used to assess the impact of both MYB factors on the steady-state level of glucosinolate biosynthetic genes and accumulation of aliphatic glucosinolates. • Both HAG2/MYB76 and HAG3/MYB29 were shown to be positive regulators of aliphatic glucosinolate biosynthesis. Expression of promoter-β- glucuronidase (GUS) fusions indicated GUS activities in both vegetative and generative organs, with distinct characteristics for each MYB factor. HAG1/MYB28, HAG2/MYB76 and HAG3/MYB29 reciprocally transactivated each other in the control of aliphatic glucosinolate biosynthesis and downregulated the expression of genes involved in the control of indolic glucosinolate biosynthesis, pointing to a reciprocal negative regulation of these two pathways. • All three HAG transcription factors exert a coordinated control on aliphatic glucosinolate biosynthesis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Methyl jasmonate-responsive genes
- Glucosinolates
- MYB transcription factors
- Regulatory network
- Transactivation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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