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Sökning: WFRF:(Hidema Jun) > UV-B-induced DNA da...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003486naa a2200409 4500
001oai:DiVA.org:oru-4152
003SwePub
008071106s2001 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-41522 URI
024a https://doi.org/10.1046/j.1365-3040.2001.00748.x2 DOI
040 a (SwePub)oru
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Kalbin, Georgiu Örebro universitet,Institutionen för naturvetenskap,Molekylär biokemi4 aut0 (Swepub:oru)gikn
2451 0a UV-B-induced DNA damage and expression of defence genes under UV-B stress :b tissue-specific molecular marker analysis in leaves
264 c 2008-10-09
264 1b Wiley,c 2001
338 a print2 rdacarrier
520 a The aim of this study was to investigate the regulatory effect of ultraviolet-B (UV-B) radiation on a number of key stress response genes found in the epidermis and mesophyll of Pisum sativum L., Argenteum mutant. This mutant was chosen for the ease with which the entire epidermis can be removed from the mesophyll tissue. An additional goal was to explore the potential modifying effect of pre-acclimation of plants to UV-B radiation prior to exposure by UV-B during treatment. Results showed that mRNA accumulation was similar during acute short-term UV-B exposure for chalcone synthase (Chs) and short-chain alcohol dehydrogenase (SadA) in both epidermis and mesophyll. In contrast, the mRNA levels differed considerably between tissues for phenylalanine ammonia lyase, chalcone isomerase and lipid transfer protein. After 24 h incubation in visible light after cessation of UV-B exposure, the regulation of mRNA levels also differed between Chs and SadA, the former showing no expression in the epidermis and the latter none in the mesophyll. Acclimation to low UV-B levels before acute exposures resulted in delayed induction of Chs and SadA. Measurements of UV-B-induced cyclobutane pyrimidine dimers (CPDs) showed a greater formation in epidermis than in mesophyll. In addition, acclimation at low UV-B levels resulted in significantly higher basal levels of CPDs than in non-acclimated plants in both mesophyll and epidermis and also in increased damage in concomitant acute exposures. The lack of correlation between the number of CPDs and levels of transcripts for defence genes, indicates that DNA damage does not control transcription of these genes.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653 a Biochemistry
653 a Biokemi
653 a Biochemistry
653 a Biokemi
700a Hidema, Jun4 aut
700a Brosché, Mikael4 aut
700a Kumagai, Tadashi4 aut
700a Bornman, Janet F.4 aut
700a Strid, Åkeu Örebro universitet,Institutionen för naturvetenskap,Molekylär biokemi4 aut0 (Swepub:oru)aesd
710a Örebro universitetb Institutionen för naturvetenskap4 org
773t Plant, Cell and Environmentd : Wileyg 24:9, s. 983-990q 24:9<983-990x 0140-7791x 1365-3040
856u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-3040.2001.00748.x
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-4152
8564 8u https://doi.org/10.1046/j.1365-3040.2001.00748.x

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