Integrated automated particle tracking microfluidic enables high-throughput cell deformability cytometry for red cell disorders

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Fältnamn	Indikatorer	Metadata
000		03343naa a2200373 4500
001		oai:DiVA.org:umu-155756
003		SwePub
008		190128s2019 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1557562 URI
024	7	a https://doi.org/10.1002/ajh.253452 DOI
040		a (SwePub)umu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Guruprasad, Puneeth4 aut
245	1 0	a Integrated automated particle tracking microfluidic enables high-throughput cell deformability cytometry for red cell disorders
264		c 2018-11-28
264	1	b Wiley-Blackwell,c 2019
338		a print2 rdacarrier
520		a Investigating individual red blood cells (RBCs) is critical to understanding hematologic diseases, as pathology often originates at the single-cell level. Many RBC disorders manifest in altered biophysical properties, such as deformability of RBCs. Due to limitations in current biophysical assays, there exists a need for high-throughput analysis of RBC deformability with single-cell resolution. To that end, we present a method that pairs a simple in vitro artificial microvasculature network system with an innovative MATLAB-based automated particle tracking program, allowing for high-throughput, single-cell deformability index (sDI) measurements of entire RBC populations. We apply our technology to quantify the sDI of RBCs from healthy volunteers, Sickle cell disease (SCD) patients, a transfusion-dependent beta thalassemia major patient, and in stored packed RBCs (pRBCs) that undergo storage lesion over 4 weeks. Moreover, our system can also measure cell size for each RBC, thereby enabling 2D analysis of cell deformability vs cell size with single cell resolution akin to flow cytometry. Our results demonstrate the clear existence of distinct biophysical RBC subpopulations with high interpatient variability in SCD as indicated by large magnitude skewness and kurtosis values of distribution, the "shifting" of sDI vs RBC size curves over transfusion cycles in beta thalassemia, and the appearance of low sDI RBC subpopulations within 4 days of pRBC storage. Overall, our system offers an inexpensive, convenient, and high-throughput method to gauge single RBC deformability and size for any RBC population and has the potential to aid in disease monitoring and transfusion guidelines for various RBC disorders.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Hematologi0 (SwePub)302022 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Hematology0 (SwePub)302022 hsv//eng
700	1	a Mannino, Robert G.4 aut
700	1	a Caruso, Christina4 aut
700	1	a Zhang, Hanqingu Umeå universitet,Institutionen för fysik4 aut0 (Swepub:umu)hazh0006
700	1	a Josephson, Cassandra D.4 aut
700	1	a Roback, John D.4 aut
700	1	a Lam, Wilbur A.4 aut
710	2	a Umeå universitetb Institutionen för fysik4 org
773	0	t American Journal of Hematologyd : Wiley-Blackwellg 94:2, s. 189-199q 94:2<189-199x 0361-8609x 1096-8652
856	4	u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ajh.25345
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-155756
856	4 8	u https://doi.org/10.1002/ajh.25345

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Av författaren/redakt...: Guruprasad, Pune ...; Mannino, Robert ...; Caruso, Christin ...; Zhang, Hanqing; Josephson, Cassa ...; Roback, John D.; visa fler...; Lam, Wilbur A.; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Hematologi

Artiklar i publikationen: American Journal ...

Av lärosätet: Umeå universitet

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