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Rough-Form Lipopolysaccharide Increases Apoptosis in Human CD4+and CD8+T Lymphocytes
- Nielsen, J. S. (author)
-
- Larsson, Anders (author)
- Uppsala universitet,Anestesiologi och intensivvård
- Ledet, T. (author)
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- Turina, M. (author)
- Tonnesen, E. (author)
- Krog, J. (author)
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- (creator_code:org_t)
- 2012-01-10
- 2012
- English.
- In: Scandinavian Journal of Immunology. - : Wiley. - 0300-9475 .- 1365-3083. ; 75:2, s. 193-202
- Journal article (peer-reviewed)
Abstract
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- Immunosuppression induced by lymphocyte apoptosis is considered an important factor in the pathogenesis of sepsis and has been demonstrated in both animal models of lipopolysaccharide (LPS)-induced endotoxemia and septic patients. As rough-form LPS (R-LPS) has recently been shown to elicit a stronger immunological response than regular smooth-form LPS (S-LPS), we aimed to assess the apoptosis-inducing capabilities of R-LPS in different subsets of lymphocytes (CD4+ T cells, CD8+ T cell, B cells and NK cells). Using multicolour flow cytometry on human peripheral blood mononuclear cells, we found that R-LPS increased apoptosis in CD4+ and CD8+ T cells assessed by annexin V and propidium iodide (AV+PI-), compared with both S-LPS-stimulated and unstimulated cells. 7-Amino-actinomycin D and staining for intracellular active caspase-3, which are considered later signs of apoptosis, did not reveal the same results. Both forms appeared to inhibit apoptosis in B cells, but no LPS-form-specific effect was seen on B or NK cells. Our results indicate that R-LPS induces a stronger AV+PI--assessed apoptotic response in T cells than S-LPS. Our findings emphasize the importance of T cell apoptosis in endotoxemia and advocates for control of LPS form in both endotoxemia research and clinical trials with Gram-negative infections.
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- Larsson, Anders
- Ledet, T.
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