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Assessment of glyco...
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Lidholt, KerstinUppsala universitet,Institutionen för medicinsk och fysiologisk kemi
(författare)
Assessment of glycosaminoglycan-protein linkage tetrasaccharides as acceptors for GalNAc- and GlcNAc-transferases from mouse mastocytoma.
- Artikel/kapitelEngelska1997
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Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-52355
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-52355URI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Two glycosaminoglycan-protein linkage tetrasaccharide-serine compounds, GlcA beta 1-3Gal beta 1-3Gal beta 1-4Xyl beta 1-O-Ser and GlcA beta 1-3Gal(4-O-sulfate)beta 1-3Gal beta 1-4Xyl beta 1-O-Ser, were tested as hexosamine accepters, using UDP-[H-3]GlcNAc and UDP-[H-3]GalNAc as sugar donors, and solubilized mouse mastocytoma microsomes as enzyme source. The nonsulfated Ser-tetrasaccharide was found to function as an acceptor for a GalNAc residue, whereas the Ser-tetrasaccharide containing a sulfated galactose unit was inactive. Characterization of the radio-labelled product by digestion with alpha-N-acetylgalactosaminidase and beta-N-acetylhexosaminidase revealed that the [H-3]GalNAc unit was alpha-linked, as in the product previously synthesized using serum enzymes, and not beta-linked as found in the chondroitin sulfate polymer. Heparan sulfate/heparin biosynthesis could not be primed by either of the two linkage Ser-tetrasaccharides, since no transfer of [H-3]GlcNAc from UDP-[H-3]GlcNAc could be detected. By contrast, transfer of a [H-3]GlcNAc unit to a [GlcA beta 1-4GlcNAca1-4](2)-GlcA beta 1-4-aMan hexasaccharide acceptor used to assay the GlcNAc transferase involved in chain elongation, was readily detected. These results are in agreement with the recent proposal that two different N-acetylglucosaminyl transferases catalyse the biosynthesis of heparan sulfate. Although the mastocytoma system contains both the heparan sulfate/heparin and chondroitin sulfate biosynthetic enzymes the Ser-tetrasaccharides do not seem to fulfil the requirements to serve as accepters for the first HexNAc transfer reactions involved in the formation of these polysaccharides.
Ämnesord och genrebeteckningar
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Biosynthesis
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GalNAc transferase
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GlcNAc transferase
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glycosaminoglycan
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proteoglycan
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Fjelstad, MariaUppsala universitet,Institutionen för medicinsk och fysiologisk kemi
(författare)
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Lindahl, UlfUppsala universitet,Institutionen för medicinsk och fysiologisk kemi
(författare)
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Goto, F
(författare)
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Ogawa, T
(författare)
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Kitagawa, H
(författare)
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Sugahara, K
(författare)
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Uppsala universitetInstitutionen för medicinsk och fysiologisk kemi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Glycoconjugate Journal14:6, s. 737-7420282-00801573-4986
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