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Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture
- Carlsson, IB (author)
- Laitinen, MPE (author)
- Scott, JE (author)
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- Louhio, H (author)
- Velentzis, L (author)
- Tuuri, T (author)
- Aaltonen, J (author)
- Ritvos, O (author)
- Winston, RML (author)
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- (creator_code:org_t)
- Bioscientifica, 2006
- 2006
- English.
- In: Reproduction (Cambridge, England). - : Bioscientifica. - 1470-1626 .- 1741-7899. ; 131:4, s. 641-649
- Journal article (peer-reviewed)
Abstract
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- The receptor tyrosine c-Kit and its cognate ligand, c-Kit ligand (KL, stem cell factor, SCF), are involved in ovarian follicular development in several animal species. We studied the expression of KL and c-Kit usingin situhybridization and immunohistochemistry in donated human ovarian cortical tissue. The KL transcripts were expressed in granulosa cells of primary follicles, whereas the expression of c-Kit was confined to the oocyte and granulosa cells in primary and secondary follicles. We employed an ovarian organ culture using firstly serum-containing and then serum-free medium to study the effects of KL and an anti-c-Kit antibody, ACK2, on the development and survival of ovarian folliclesin vitro. Culture of ovarian cortical slices for 7 days resulted in a 37% increase in the number of primary follicles and a 6% increase in secondary follicles. The proportion of viable follicles decreased in all cultures. The addition of KL (1, 10 and 100 ng/ml) into the culture media did not affect the developmental stages of the follicles or the proportion of atretic follicles. Inclusion of ACK2 (800 ng/ml) in the culture medium significantly increased the proportion of atretic follicles on days 7 (49 vs 28% in control cultures) and 14 (62 vs 38%) of culture. In conclusion, c-Kit and KL are expressed in human ovaries during follicular development. Blocking the c-Kit receptor induces follicular atresia. The KL/c-Kit signaling system is likely to control the survival of human ovarian follicles during early follicular development.
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- Carlsson, IB
- Laitinen, MPE
- Scott, JE
- Louhio, H
- Velentzis, L
- Tuuri, T
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- Aaltonen, J
- Ritvos, O
- Winston, RML
- Hovatta, O
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