Enhanced anti-tumor immune responses and delay of tumor development in human epidermal growth factor receptor 2 mice immunized with an immunostimulatory peptide in poly(D, L-lactic-co-glycolic) acid nanoparticles nanoparticles

Larsson, Marie (author): Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Hälsouniversitetet

(creator_code:org_t)

2015-03-31
2015
English.
In: Breast Cancer Research. - : BioMed Central. - 1465-5411 .- 1465-542X. ; 17:48

Related links:: https://liu.diva-por... (primary) (Raw object); show more...; https://breast-cance...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

Introduction Cancer vaccines have the potential to induce curative anti-tumor immune responses and better adjuvants may improve vaccine efficacy. We have previously shown that Hp91, a peptide derived from the B box domain in high-mobility group box protein 1 (HMGB1), acts as potent immune adjuvant. Method In this study, Hp91 was tested as part of a therapeutic vaccine against human epidermal growth factor receptor 2 (HER2) positive breast cancer. Results Free peptide did not significantly augment immune responses but, when delivered in poly(D, L-lactic-co-glycolic) acid nanoparticles (PLGA-NPs), robust activation of dendritic cells (DCs) and increased activation of HER2 specific T cells was observed in vitro. Vaccination of HER2NEU transgenic mice, a mouse breast cancer model that closely mimics the immune modulation and tolerance in some breast cancer patients, with Hp91 loaded PLGA-NPs enhanced the activation of HER2 specific cytotoxic T lymphocyte (CTL) responses, delayed tumor development, and prolonged survival. Conclusion Taken together these findings demonstrate that the delivery of the immunostimulatory peptide Hp91 inside PLGA-NPs enhances the potency of the peptide and efficacy of a breast cancer vaccine.

By the author/editor: Campbell, Diahnn; Saenz, Rebecca; Bharati, Ila S.; Seible, Daniel; Zhang, Liangfang; Esener, Sadik; show more...; Messmer, Bradley; Larsson, Marie; Messmer, Davorka; show less...

About the subject

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