Sökning: WFRF:(Partridge Bruce) > (2009) > A global benchmark ...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 08021naa a2202089 4500 | |
001 | oai:DiVA.org:sh-13911 | |
003 | SwePub | |
008 | 111214s2009 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-139112 URI |
024 | 7 | a https://doi.org/10.1016/j.ab.2008.11.0212 DOI |
040 | a (SwePub)sh | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Rich, Rebecca L.4 aut |
245 | 1 0 | a A global benchmark study using affinity-based biosensors |
264 | 1 | b Elsevier BV,c 2009 |
338 | a print2 rdacarrier | |
520 | a To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
700 | 1 | a Papalia, Giuseppe A.4 aut |
700 | 1 | a Flynn, Peter J.4 aut |
700 | 1 | a Furneisen, Jamie4 aut |
700 | 1 | a Quinn, John4 aut |
700 | 1 | a Klein, Joshua S.4 aut |
700 | 1 | a Katsamba, Phini S.4 aut |
700 | 1 | a Waddell, M. Brent4 aut |
700 | 1 | a Scott, Michael4 aut |
700 | 1 | a Thompson, Joshua4 aut |
700 | 1 | a Berlier, Judie4 aut |
700 | 1 | a Corry, Schuyler4 aut |
700 | 1 | a Baltzinger, Mireille4 aut |
700 | 1 | a Zeder-Lutzi, Gabrielle4 aut |
700 | 1 | a Schoenemann, Andreas4 aut |
700 | 1 | a Clabbers, Anca4 aut |
700 | 1 | a Wieckowski, Sebastien4 aut |
700 | 1 | a Murphy, Mary M.4 aut |
700 | 1 | a Page, Phillip4 aut |
700 | 1 | a Ryan, Thomas E.4 aut |
700 | 1 | a Duffner, Jay4 aut |
700 | 1 | a Ganguly, Tanmoy4 aut |
700 | 1 | a Corbin, John4 aut |
700 | 1 | a Gautam, Satyen4 aut |
700 | 1 | a Anderluh, Gregor4 aut |
700 | 1 | a Bavdek, Andrej4 aut |
700 | 1 | a Reichmann, Dana4 aut |
700 | 1 | a Yadav, Satya P.4 aut |
700 | 1 | a Hommema, Eric4 aut |
700 | 1 | a Pol, Ewa4 aut |
700 | 1 | a Drake, Andrew4 aut |
700 | 1 | a Klakamp, Scott4 aut |
700 | 1 | a Chapman, Trevor4 aut |
700 | 1 | a Kernaghan, Dawn4 aut |
700 | 1 | a Miller, Ken4 aut |
700 | 1 | a Schuman, Jason4 aut |
700 | 1 | a Lindquist, Kevin4 aut |
700 | 1 | a Herlihy, Kara4 aut |
700 | 1 | a Murphy, Michael B.4 aut |
700 | 1 | a Bohnsack, Richard4 aut |
700 | 1 | a Andrien, Bruce4 aut |
700 | 1 | a Brandani, Pietro4 aut |
700 | 1 | a Terwey, Danny4 aut |
700 | 1 | a Millican, Rohn4 aut |
700 | 1 | a Darling, Ryan J.4 aut |
700 | 1 | a Wang, Liann4 aut |
700 | 1 | a Carter, Quincy4 aut |
700 | 1 | a Dotzlaf, Joe4 aut |
700 | 1 | a Lopez-Sagaseta, Jacinto4 aut |
700 | 1 | a Campbell, Islay4 aut |
700 | 1 | a Torreri, Paola4 aut |
700 | 1 | a Hoos, Sylviane4 aut |
700 | 1 | a England, Patrick4 aut |
700 | 1 | a Liu, Yang4 aut |
700 | 1 | a Abdiche, Yasmina4 aut |
700 | 1 | a Malashock, Daniel4 aut |
700 | 1 | a Pinkerton, Alanna4 aut |
700 | 1 | a Wong, Melanie4 aut |
700 | 1 | a Lafer, Eileen4 aut |
700 | 1 | a Hinck, Cynthia4 aut |
700 | 1 | a Thompson, Kevin4 aut |
700 | 1 | a Di Primo, Carmelo4 aut |
700 | 1 | a Joyce, Alison4 aut |
700 | 1 | a Brooks, Jonathan4 aut |
700 | 1 | a Torta, Federico4 aut |
700 | 1 | a Hagel, Anne Birgitte Bagge4 aut |
700 | 1 | a Krarup, Janus4 aut |
700 | 1 | a Pass, Jesper4 aut |
700 | 1 | a Ferreira, Monicau Södertörns högskola,Molekylärbiologi4 aut0 (Swepub:sh)SHMAFA |
700 | 1 | a Shikov, Sergei4 aut |
700 | 1 | a Mikolajczyk, Malgorzata4 aut |
700 | 1 | a Abe, Yuki4 aut |
700 | 1 | a Barbato, Gaetano4 aut |
700 | 1 | a Giannetti, Anthony M.4 aut |
700 | 1 | a Krishnamoorthy, Ganeshram4 aut |
700 | 1 | a Beusink, Bianca4 aut |
700 | 1 | a Satpaev, Daulet4 aut |
700 | 1 | a Tsang, Tiffany4 aut |
700 | 1 | a Fang, Eric4 aut |
700 | 1 | a Partridge, James4 aut |
700 | 1 | a Brohawn, Stephen4 aut |
700 | 1 | a Horn, James4 aut |
700 | 1 | a Pritsch, Otto4 aut |
700 | 1 | a Obal, Gonzalo4 aut |
700 | 1 | a Nilapwar, Sanjay4 aut |
700 | 1 | a Busby, Ben4 aut |
700 | 1 | a Gutierrez-Sanchez, Gerardo4 aut |
700 | 1 | a Das Gupta, Ruchira4 aut |
700 | 1 | a Canepa, Sylvie4 aut |
700 | 1 | a Witte, Krista4 aut |
700 | 1 | a Nikolovska-Coleska, Zaneta4 aut |
700 | 1 | a Cho, Yun Hee4 aut |
700 | 1 | a D'Agata, Roberta4 aut |
700 | 1 | a Schlick, Kristian4 aut |
700 | 1 | a Calvert, Rosy4 aut |
700 | 1 | a Munoz, Eva M.4 aut |
700 | 1 | a Hernaiz, Maria Jose4 aut |
700 | 1 | a Bravman, Tsafir4 aut |
700 | 1 | a Dines, Monica4 aut |
700 | 1 | a Yang, Min-Hsiang4 aut |
700 | 1 | a Puskas, Agnes4 aut |
700 | 1 | a Boni, Erica4 aut |
700 | 1 | a Li, Jiejin4 aut |
700 | 1 | a Wear, Martin4 aut |
700 | 1 | a Grinberg, Asya4 aut |
700 | 1 | a Baardsnes, Jason4 aut |
700 | 1 | a Dolezal, Olan4 aut |
700 | 1 | a Gainey, Melicia4 aut |
700 | 1 | a Anderson, Henrik4 aut |
700 | 1 | a Peng, Jinlin4 aut |
700 | 1 | a Lewis, Mark4 aut |
700 | 1 | a Spies, Peter4 aut |
700 | 1 | a Trinh, Quyhn4 aut |
700 | 1 | a Bibikov, Sergei4 aut |
700 | 1 | a Raymond, Jill4 aut |
700 | 1 | a Yousef, Mohammed4 aut |
700 | 1 | a Chandrasekaran, Vidya4 aut |
700 | 1 | a Feng, Yuguo4 aut |
700 | 1 | a Emerick, Anne4 aut |
700 | 1 | a Mundodo, Suparna4 aut |
700 | 1 | a Guimaraes, Rejane4 aut |
700 | 1 | a McGirr, Katy4 aut |
700 | 1 | a Li, Yue-Ji4 aut |
700 | 1 | a Hughes, Heather4 aut |
700 | 1 | a Mantz, Hubert4 aut |
700 | 1 | a Skrabana, Rostislav4 aut |
700 | 1 | a Witmer, Mark4 aut |
700 | 1 | a Ballard, Joshua4 aut |
700 | 1 | a Martin, Loic4 aut |
700 | 1 | a Skladal, Petr4 aut |
700 | 1 | a Korza, George4 aut |
700 | 1 | a Laird-Offringa, Ite4 aut |
700 | 1 | a Lee, Charlene S.4 aut |
700 | 1 | a Khadir, Abdelkrim4 aut |
700 | 1 | a Podlaski, Frank4 aut |
700 | 1 | a Neuner, Phillippe4 aut |
700 | 1 | a Rothacker, Julie4 aut |
700 | 1 | a Rafique, Ashique4 aut |
700 | 1 | a Dankbar, Nico4 aut |
700 | 1 | a Kainz, Peter4 aut |
700 | 1 | a Gedig, Erk4 aut |
700 | 1 | a Vuyisich, Momchilo4 aut |
700 | 1 | a Boozer, Christina4 aut |
700 | 1 | a Ly, Nguyen4 aut |
700 | 1 | a Toews, Mark4 aut |
700 | 1 | a Uren, Aykut4 aut |
700 | 1 | a Kalyuzhniy, Oleksandr4 aut |
700 | 1 | a Lewis, Kenneth4 aut |
700 | 1 | a Chomey, Eugene4 aut |
700 | 1 | a Pak, Brian J.4 aut |
700 | 1 | a Myszka, David G.4 aut |
710 | 2 | a Södertörns högskolab Molekylärbiologi4 org |
773 | 0 | t Analytical Biochemistryd : Elsevier BVg 386:2, s. 194-216q 386:2<194-216x 0003-2697x 1096-0309 |
856 | 4 | u https://europepmc.org/articles/pmc3793259?pdf=render |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-13911 |
856 | 4 8 | u https://doi.org/10.1016/j.ab.2008.11.021 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.