WFRF:(Rocha Martín Javier)
Sökning: WFRF:(Rocha Martín Javier) > (2008-2009) > Coating of soluble ...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04009naa a2200385 4500 | |
| 001 | oai:DiVA.org:umu-81863 | |
| 003 | SwePub | |
| 008 | 131022s2009 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-818632 URI |
| 024 | 7 | a https://doi.org/10.1021/bm801162e2 DOI |
| 040 | a (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Bolivar, Juan Mu Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Cantoblanco, Madrid, Spain4 aut |
| 245 | 1 0 | a Coating of soluble and immobilized enzymes with ionic polymers :b full stabilization of the quaternary structure of multimeric enzymes |
| 264 | c 2009-03-06 | |
| 264 | 1 | b American Chemical Society (ACS),c 2009 |
| 338 | a print2 rdacarrier | |
| 520 | a This paper shows a simple and effective way to avoid the dissociation of multimeric enzymes by coating their surface with a large cationic polymer (e.g., polyethylenimine (PEI)) by ionic exchange. As model enzymes, glutamate dehydrogenase (GDH) from Thermus thermophilus and formate dehydrogenase (FDH) from Pseudomonas sp. were used. Both enzymes are very unstable at acidic pH values due to the rapid dissociation of their subunits (half-life of diluted preparations is few minutes at pH 4 and 25 degrees C). GDH and FDH were incubated in the presence of PEI yielding an enzyme-PEI composite with full activity. To stabilize the enzyme-polymer composite, a treatment with glutaraldehyde was required. These enzyme-PEI composites can be crosslinked with glutaraldehyde by immobilizing previously the composite onto a weak cationic exchanger. The soluble GDH-PEI composite was much more stable than unmodified GDH at pH 4 and 30 degrees C (retaining over 90% activity after 24 h incubation) with no effect of the GDH concentration in the inactivation course. The composite could be very strongly, but reversibly, adsorbed on cationic exchangers. Similarly, FDH could be treated with PEI and glutaraldehyde after adsorption on cationic exchangers, This permitted a stabilized FDH preparation. In this way, the coating of the enzymes surfaces with PEI is used as a simple and efficient strategy to prevent enzyme dissociation of multimeric enzymes. These composites can be used as a soluble catalyst or reversibly immobilized onto a cationic exchanger (e.g., CM-agarose). | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Kemix Polymerkemi0 (SwePub)104062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Chemical Sciencesx Polymer Chemistry0 (SwePub)104062 hsv//eng |
| 700 | 1 | a Rocha-Martin, Javieru Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Cantoblanco, Madrid, Spain4 aut |
| 700 | 1 | a Mateo, Cesaru Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Cantoblanco, Madrid, Spain4 aut |
| 700 | 1 | a Cava, Felipeu CBM ‘Severo Ochoa’ CSIC-UAM, Madrid, Spain4 aut0 (Swepub:umu)feca0003 |
| 700 | 1 | a Berenguer, Joseu CBM ‘Severo Ochoa’ CSIC-UAM, Madrid, Spain4 aut |
| 700 | 1 | a Fernandez-Lafuente, Robertou Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Cantoblanco, Madrid, Spain4 aut |
| 700 | 1 | a Guisan, Jose Mu Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Cantoblanco, Madrid, Spain4 aut |
| 710 | 2 | a Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Cantoblanco, Madrid, Spainb CBM ‘Severo Ochoa’ CSIC-UAM, Madrid, Spain4 org |
| 773 | 0 | t Biomacromoleculesd : American Chemical Society (ACS)g 10:4, s. 742-747q 10:4<742-747x 1525-7797x 1526-4602 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-81863 |
| 856 | 4 8 | u https://doi.org/10.1021/bm801162e |
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