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Determination of Le...
Determination of Lewis FUT3 gene mutations by PCR using sequence-specific primers enables efficient genotyping of clinical samples.
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- Grahn, Ammi, 1961 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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Elmgren, A (author)
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Aberg, L (author)
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- Svensson, Lola, 1948 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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Jansson, Per-Anders, 1961 (author)
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Lönnroth, Peter, 1951 (author)
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- Larson, Göran, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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(creator_code:org_t)
- 2001-09-18
- 2001
- English.
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In: Human mutation. - : Hindawi Limited. - 1098-1004 .- 1059-7794. ; 18:4, s. 358-9
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We have developed a polymerase chain reaction method using sequence-specific primers (PCR-SSP) for rapid and correct genotyping of the common Lewis (FUT3) gene mutations 59T>G, 202T>C, 314C>T, 508G>A, and 1067T>A. The PCR-SSP method was validated on 20 healthy blood donors and 16 non-insulin-dependent diabetic patients. All individuals were in parallel genotyped by our established polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The FUT3 genotypes, determined with the PCR-SSP method, were in complete accordance with the results of the PCR-RFLP reference method. The PCR-SSP method could also be adapted to assign the presence of a specific mutation to the respective FUT3 alleles. We found the method to be reliable, rapid and cheap with no requirements for restriction enzyme processing.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
Keyword
- Alleles
- DNA Mutational Analysis
- methods
- DNA Primers
- genetics
- Diabetes Mellitus
- Type 2
- enzymology
- genetics
- Fucosyltransferases
- genetics
- Genetic Testing
- methods
- Genotype
- Hemagglutination Tests
- Humans
- Mutation
- genetics
- Polymerase Chain Reaction
- methods
- Polymorphism
- Restriction Fragment Length
- Reproducibility of Results
- Sensitivity and Specificity
- Substrate Specificity
- Time Factors
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Grahn, Ammi, 196 ...
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Elmgren, A
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Aberg, L
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Svensson, Lola, ...
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Jansson, Per-And ...
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Lönnroth, Peter, ...
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show more...
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Larson, Göran, 1 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Other Clinical M ...
- Articles in the publication
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Human mutation
- By the university
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University of Gothenburg