Sökning: WFRF:(Thor Andreas) > Invisible detergent...
Fältnamn | Indikatorer | Metadata |
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000 | 03386naa a2200541 4500 | |
001 | oai:lup.lub.lu.se:35ac359d-bd09-4b43-a41b-af57403f3534 | |
003 | SwePub | |
008 | 171215s2018 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/35ac359d-bd09-4b43-a41b-af57403f35342 URI |
024 | 7 | a https://doi.org/10.1111/febs.143452 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Midtgaard, Søren Roi4 aut |
245 | 1 0 | a Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering |
264 | c 2017-12-30 | |
264 | 1 | b Wiley,c 2018 |
520 | a A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved. | |
700 | 1 | a Darwish, Tamim A4 aut |
700 | 1 | a Pedersen, Martin Cramer4 aut |
700 | 1 | a Huda, Pie4 aut |
700 | 1 | a Larsen, Andreas Haahr4 aut |
700 | 1 | a Jensen, Grethe Vestergaard4 aut |
700 | 1 | a Kynde, Søren Andreas Røssel4 aut |
700 | 1 | a Skar-Gislinge, Nicholas4 aut |
700 | 1 | a Nielsen, Agnieszka Janina Zygadlo4 aut |
700 | 1 | a Olesen, Claus4 aut |
700 | 1 | a Blaise, Mickael4 aut |
700 | 1 | a Dorosz, Jerzy Józef4 aut |
700 | 1 | a Thorsen, Thor Seneca4 aut |
700 | 1 | a Venskutonytė, Ramintau Lund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups4 aut0 (Swepub:lu)ra5746ve |
700 | 1 | a Krintel, Christianu University of Copenhagen4 aut0 (Swepub:lu)med-ctk |
700 | 1 | a Møller, Jesper V4 aut |
700 | 1 | a Frielinghaus, Henrich4 aut |
700 | 1 | a Gilbert, Elliot Paul4 aut |
700 | 1 | a Martel, Anne4 aut |
700 | 1 | a Kastrup, Jette Sandholm4 aut |
700 | 1 | a Jensen, Poul Erik4 aut |
700 | 1 | a Nissen, Poul4 aut |
700 | 1 | a Arleth, Lise4 aut |
710 | 2 | a Medicinsk strukturbiologib Forskargrupper vid Lunds universitet4 org |
773 | 0 | t The FEBS Journald : Wileyg 285:2, s. 357-371q 285:2<357-371x 1742-464Xx 1742-4658 |
856 | 4 | u http://dx.doi.org/10.1111/febs.14345y FULLTEXT |
856 | 4 | u https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/febs.14345 |
856 | 4 8 | u https://lup.lub.lu.se/record/35ac359d-bd09-4b43-a41b-af57403f3534 |
856 | 4 8 | u https://doi.org/10.1111/febs.14345 |
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