Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering

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Fältnamn	Indikatorer	Metadata
000		03386naa a2200541 4500
001		oai:lup.lub.lu.se:35ac359d-bd09-4b43-a41b-af57403f3534
003		SwePub
008		171215s2018 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://lup.lub.lu.se/record/35ac359d-bd09-4b43-a41b-af57403f35342 URI
024	7	a https://doi.org/10.1111/febs.143452 DOI
040		a (SwePub)lu
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Midtgaard, Søren Roi4 aut
245	1 0	a Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering
264		c 2017-12-30
264	1	b Wiley,c 2018
520		a A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2 O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins by SANS and subsequent data analysis available to non-specialists. This article is protected by copyright. All rights reserved.
700	1	a Darwish, Tamim A4 aut
700	1	a Pedersen, Martin Cramer4 aut
700	1	a Huda, Pie4 aut
700	1	a Larsen, Andreas Haahr4 aut
700	1	a Jensen, Grethe Vestergaard4 aut
700	1	a Kynde, Søren Andreas Røssel4 aut
700	1	a Skar-Gislinge, Nicholas4 aut
700	1	a Nielsen, Agnieszka Janina Zygadlo4 aut
700	1	a Olesen, Claus4 aut
700	1	a Blaise, Mickael4 aut
700	1	a Dorosz, Jerzy Józef4 aut
700	1	a Thorsen, Thor Seneca4 aut
700	1	a Venskutonytė, Ramintau Lund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups4 aut0 (Swepub:lu)ra5746ve
700	1	a Krintel, Christianu University of Copenhagen4 aut0 (Swepub:lu)med-ctk
700	1	a Møller, Jesper V4 aut
700	1	a Frielinghaus, Henrich4 aut
700	1	a Gilbert, Elliot Paul4 aut
700	1	a Martel, Anne4 aut
700	1	a Kastrup, Jette Sandholm4 aut
700	1	a Jensen, Poul Erik4 aut
700	1	a Nissen, Poul4 aut
700	1	a Arleth, Lise4 aut
710	2	a Medicinsk strukturbiologib Forskargrupper vid Lunds universitet4 org
773	0	t The FEBS Journald : Wileyg 285:2, s. 357-371q 285:2<357-371x 1742-464Xx 1742-4658
856	4	u http://dx.doi.org/10.1111/febs.14345y FULLTEXT
856	4	u https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/febs.14345
856	4 8	u https://lup.lub.lu.se/record/35ac359d-bd09-4b43-a41b-af57403f3534
856	4 8	u https://doi.org/10.1111/febs.14345

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