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Characterization of...
Characterization of CMVpp65-specific CD8+ T lymphocytes using MHC tetramers in kidney transplant patients and healthy participants
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- Engstrand, Mats (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,KITM
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Tournay, Claire (författare)
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Peyrat, Marie A. (författare)
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- Eriksson, Britt-Marie (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Friman, infektion
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- Wadström, Jonas (författare)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper,Transplantation Surgery
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- Zweygberg Wirgart, Benita (författare)
- Karolinska Institutet
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Romagné, Francois (författare)
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Bonneville, Marc (författare)
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- Tötterman, Thomas H. (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,KITM
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- Korsgren, Olle (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,KITM
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(creator_code:org_t)
- Ovid Technologies (Wolters Kluwer Health), 2000
- 2000
- Engelska.
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Ingår i: Transplantation. - : Ovid Technologies (Wolters Kluwer Health). - 0041-1337 .- 1534-6080. ; 69:11, s. 2243-2250
- Relaterad länk:
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https://urn.kb.se/re...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- BACKGROUND: Cytomegalovirus (CMV) is a ubiquitous herpesvirus that infects 50-90% of individuals in different populations. After primary infection, the virus persists latently in myeloid cells under the control of specific T-cells. Reactivation of CMV infection may cause lethal organ dysfunction and is frequently seen in immunosuppressed individuals. CD8+ cytotoxic T-cells (CTL) have a primary role in suppressing CMV reactivation, and the dominating CTL response is directed against pp65. METHODS: MHC tetramers, that is, complexes between HLA class I (or class II) molecules and antigenic peptides conjugated to fluorochromes allow the direct visualization of antigen-specific receptor-carrying T-cells using flow cytometry. We constructed a novel MHC tetramer for identification of CMVpp65-specific CD8+ T-cells using HLA-A2 molecules folded with the immunodominant NLVPMVATV peptide. RESULTS: The A2/pp65 tetramer specifically stained CMV-directed T-cell lines, and sorted cells showed CMV-specific cytotoxicity. High proportions (0.1-9%) of the CD8+ T-cells were A2/pp65 tetramer+ in healthy HLA-A2+ CMV carriers and in immunosuppressed kidney transplant patients with latent infection. Patients with reactivated CMV infection exhibited up to 15% A2/pp65 tetramer+ cells, which seemed to correlate with CMV load over time. A2/pp65 tetramer+ cells expressed T-cell activation markers. CONCLUSIONS: The construction of a novel A2/pp65 MHC tetramer enabled the design of a rapid and precise flow cytometric method allowing quantitative and qualitative analysis of CMV-specific T-cells. The number of A2/pp65 tetramer binding CTLs in blood may prove to be clinically relevant in assessing the immune response to CMV.
Nyckelord
- MEDICINE
- MEDICIN
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- art (ämneskategori)
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