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Sökning: WFRF:(Wollscheid Bernd) > (2012) > Proteomic Analysis ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003624naa a2200517 4500
001oai:DiVA.org:kth-104700
003SwePub
008121109s2012 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1047002 URI
0247 a https://doi.org/10.1021/pr300360a2 DOI
040 a (SwePub)kth
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Bock, Thomas4 aut
2451 0a Proteomic Analysis Reveals Drug Accessible Cell Surface N-Glycoproteins of Primary and Established Glioblastoma Cell Lines
264 c 2012-08-31
264 1b American Chemical Society (ACS),c 2012
338 a print2 rdacarrier
500 a QC 20121112
520 a Glioblastoma is the most common primary Glioblastoma Cell Surface Capturing brain tumor in adults with low average survival time after diagnosis. In order to improve glioblastoma treatment, new drug-accessible targets need to be identified. Cell surface glycoproteins are prime drug targets due to their accessibility at the surface of cancer cells. To overcome the limited availability of suitable antibodies for cell surface protein detection, we performed a comprehensive mass spectrometric investigation of the glioblastoma surfaceome. Our combined cell surface capturing analysis of primary ex vivo glioblastoma cell lines in combination with established glioblastoma cell lines revealed 633 N-glycoproteins, which vastly extends the known data of surfaceome drug targets at subcellular resolution. We provide direct evidence of common glioblastoma cell surface glycoproteins and an approximate estimate of their abundances, information that could not be derived from genomic and/or transcriptomic glioblastoma studies. Apart from our pharmaceutically valuable repertoire of already and potentially drug-accessible cell surface glycoproteins, we built a mass-spectrometry-based toolbox enabling directed, sensitive, and repetitive glycoprotein measurements for clinical follow-up studies. The included Skyline Glioblastoma SRM assay library provides an elevated starting point for parallel testing of the abundance level of the detected glioblastoma surfaceome members in future drug perturbation experiments.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653 a glioblastoma
653 a N-glycoproteins
653 a cell surface accessible drug targets
653 a cell surface capturing
653 a N-glycoproteome SRM library
7001 a Moest, Hansjoerg4 aut
7001 a Omasits, Ulrich4 aut
7001 a Dolski, Silvia4 aut
7001 a Lundberg, Emmau KTH,Proteomik,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u12bylj1
7001 a Frei, Andreas4 aut
7001 a Hofmann, Andreas4 aut
7001 a Bausch-Fluck, Damaris4 aut
7001 a Jacobs, Andrea4 aut
7001 a Krayenbuehl, Niklaus4 aut
7001 a Uhlén, Mathiasu KTH,Proteomik,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dulvmw
7001 a Aebersold, Ruedi4 aut
7001 a Frei, Karl4 aut
7001 a Wollscheid, Bernd4 aut
7102 a KTHb Proteomik4 org
7730 t Journal of Proteome Researchd : American Chemical Society (ACS)g 11:10, s. 4885-4893q 11:10<4885-4893x 1535-3893x 1535-3907
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-104700
8564 8u https://doi.org/10.1021/pr300360a

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