Sökning: WFRF:(de Luca A) > (1980-1984) > Proteoglycans from ...
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000 | 03782naa a2200301 4500 | |
001 | oai:lup.lub.lu.se:c8516e35-bb5e-4858-9792-048e1a96c58a | |
003 | SwePub | |
008 | 160504s1980 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/c8516e35-bb5e-4858-9792-048e1a96c58a2 URI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a de Luca, S.u National Institutes of Health, United States4 aut |
245 | 1 0 | a Proteoglycans from chick limb bud chondrocyte cultures. Keratan sulfate and oligosaccharides which contain mannose and sialic acid |
264 | 1 | c 1980 |
300 | a 7 s. | |
520 | a The precursors, [ 35S]sulfate and [2- 3H]mannose, were used to study the biosynthesis of keratan sulfate and other oligosaccharides on proteoglycans isolated from Day 8 cultures of chick limb bud chondrocytes. After alkaline borohydride treatment, three fractions with sialic acid were separated by molecular sieve chromatography. The first contained keratan sulfate which was purified by digestion with chondroitinase to remove chondroitin sulfate, followed by molecular sieve and ion exchange chromatography. The purified keratan sulfate contained about 8% of the 35S activity originally in monomer. The chains had an average length of about 40 monosaccharides and contained only trace amounts of mannose (less than 1 residue/three to four chains). The second fraction contained the majority of the [ 3H]mannose originally in monomer, but no 35S activity. This fraction appears to contain oligosaccharide-peptides of the asparagine-N-glycosylamine type because there were no reduced sugars present and the alkaline borohydride treatment extensively degraded the core protein. The composition of the oligosaccharides, with high proportions of mannose, N-acetylglucosamine, galactose, and sialic acid, was consistent with this suggestion. The third fraction consisted of a series of oligosaccharides with sizes between three to six saccharides. They contained N-acetylgalactosaminitol, indicating that they were attached to the core protein by O-glycoside bonds between N-acetylgalactosamine and hydroxyl groups on serine and threonine. Thus, proteoglycans contain two classes of oligosaccharides, a mannose-rich class characteristic of glycoproteins and an O-glycoside class characteristic of mucins, in addition to the chondroitin sulfate and keratan sulfate chains. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Ortopedi0 (SwePub)302112 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Orthopaedics0 (SwePub)302112 hsv//eng |
700 | 1 | a Lohmander, Stefanu Lund University,Lunds universitet,Ortopedi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund OsteoArthritis Division - Nedbrytning av ledbrosk: en biologisk process som leder till artros,Forskargrupper vid Lunds universitet,Orthopaedics (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund OsteoArthritis Division - Molecular marker research group,Lund University Research Groups4 aut0 (Swepub:lu)ort-slo |
700 | 1 | a Nilsson, Boelu Lund University,Lunds universitet,Oftalmologi, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Ophthalmology, Lund,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)oft-bni |
700 | 1 | a Hascall, V. C.4 aut |
700 | 1 | a Caplan, A. I.4 aut |
710 | 2 | a National Institutes of Health, United Statesb Ortopedi, Lund4 org |
773 | 0 | t Journal of Biological Chemistryg 255:13, s. 6077-6083q 255:13<6077-6083x 0021-9258 |
856 | 4 8 | u https://lup.lub.lu.se/record/c8516e35-bb5e-4858-9792-048e1a96c58a |
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