Search: onr:"swepub:oai:DiVA.org:kth-93008" > Identification of A...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03072naa a2200505 4500 | |
001 | oai:DiVA.org:kth-93008 | |
003 | SwePub | |
008 | 120410s2012 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-930082 URI |
024 | 7 | a https://doi.org/10.1074/mcp.M111.0140352 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Dengjel, Joern4 aut |
245 | 1 0 | a Identification of Autophagosome-associated Proteins and Regulators by Quantitative Proteomic Analysis and Genetic Screens |
264 | 1 | c 2012 |
338 | a print2 rdacarrier | |
500 | a QC 20120411 | |
520 | a Autophagy is one of the major intracellular catabolic pathways, but little is known about the composition of autophagosomes. To study the associated proteins, we isolated autophagosomes from human breast cancer cells using two different biochemical methods and three stimulus types: amino acid deprivation or rapamycin or concanamycin A treatment. The autophagosome- associated proteins were dependent on stimulus, but a core set of proteins was stimulus- independent. Remarkably, proteasomal proteins were abundant among the stimulus- independent common autophagosome- associated proteins, and the activation of autophagy significantly decreased the cellular proteasome level and activity supporting interplay between the two degradation pathways. A screen of yeast strains defective in the orthologs of the human genes encoding for a common set of autophagosome- associated proteins revealed several regulators of autophagy, including subunits of the retromer complex. The combined spatiotemporal proteomic and genetic data sets presented here provide a basis for further characterization of autophagosome biogenesis and cargo selection. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
700 | 1 | a Hoyer-Hansen, Maria4 aut |
700 | 1 | a Nielsen, Maria O.4 aut |
700 | 1 | a Eisenberg, Tobias4 aut |
700 | 1 | a Harder, Lea M.4 aut |
700 | 1 | a Schandorff, Soren4 aut |
700 | 1 | a Farkas, Thomas4 aut |
700 | 1 | a Kirkegaard, Thomas4 aut |
700 | 1 | a Becker, Andrea C.4 aut |
700 | 1 | a Schroeder, Sabrina4 aut |
700 | 1 | a Vanselow, Katja4 aut |
700 | 1 | a Lundberg, Emmau KTH,Proteomik,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u12bylj1 |
700 | 1 | a Nielsen, Mogens M.4 aut |
700 | 1 | a Kristensen, Anders R.4 aut |
700 | 1 | a Akimov, Vyacheslav4 aut |
700 | 1 | a Bunkenborg, Jakob4 aut |
700 | 1 | a Madeo, Frank4 aut |
700 | 1 | a Jaattela, Marja4 aut |
700 | 1 | a Andersen, Jens S.4 aut |
710 | 2 | a KTHb Proteomik4 org |
773 | 0 | t Molecular & Cellular Proteomicsg 11:3q 11:3x 1535-9476x 1535-9484 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-93008 |
856 | 4 8 | u https://doi.org/10.1074/mcp.M111.014035 |
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