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Sökning: onr:"swepub:oai:DiVA.org:su-196280" > Profiling of Extrac...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003660naa a2200553 4500
001oai:DiVA.org:su-196280
003SwePub
008210906s2021 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:146991633
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1962802 URI
0247 a https://doi.org/10.3390/cells100615432 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1469916332 URI
040 a (SwePub)sud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Sork, Helenau Karolinska Institutet4 aut
2451 0a Profiling of Extracellular Small RNAs Highlights a Strong Bias towards Non-Vesicular Secretion
264 c 2021-06-18
264 1b MDPI AG,c 2021
338 a print2 rdacarrier
520 a The extracellular environment consists of a plethora of molecules, including extracellular miRNA that can be secreted in association with extracellular vesicles (EVs) or soluble protein complexes (non-EVs). Yet, interest in therapeutic short RNA carriers lies mainly in EVs, the vehicles conveying the great majority of the biological activity. Here, by overexpressing miRNA and shRNA sequences in parent cells and using size exclusion liquid chromatography (SEC) to separate the secretome into EV and non-EV fractions, we saw that >98% of overexpressed miRNA was secreted within the non-EV fraction. Furthermore, small RNA sequencing studies of native miRNA transcripts revealed that although the abundance of miRNAs in EVs, non-EVs and parent cells correlated well (R-2 = 0.69-0.87), quantitatively an outstanding 96.2-99.9% of total miRNA was secreted in the non-EV fraction. Nevertheless, though EVs contained only a fraction of secreted miRNAs, these molecules were stable at 37 degrees C in a serum-containing environment, indicating that if sufficient miRNA loading is achieved, EVs can remain delivery-competent for a prolonged period of time. This study suggests that the passive endogenous EV loading strategy might be a relatively wasteful way of loading miRNA to EVs, and active miRNA loading approaches are needed for developing advanced EV miRNA therapies in the future.
650 7a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
653 a extracellular vesicles
653 a small RNA
653 a SEC
653 a extracellular RNA
653 a miRNA
7001 a Conceicao, Mariana4 aut
7001 a Corso, Giuliau Karolinska Institutet4 aut
7001 a Nordin, Joelu Karolinska Institutet4 aut
7001 a Lee, Yi Xin Fiona4 aut
7001 a Krjutskov, Kaarel4 aut
7001 a Orzechowski Westholm, Jakubu Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för biokemi och biofysik4 aut0 (Swepub:su)jaor0050
7001 a Vader, Pieter4 aut
7001 a Pauwels, Marie4 aut
7001 a Vandenbroucke, Roosmarijn E.4 aut
7001 a Wood, Matthew J. A.4 aut
7001 a EL Andaloussi, Samiru Karolinska Institutet4 aut
7001 a Mager, Imre4 aut
7102 a Karolinska Institutetb Science for Life Laboratory (SciLifeLab)4 org
7730 t Cellsd : MDPI AGg 10:6q 10:6x 2073-4409
8564 u https://doi.org/10.3390/cells10061543y Fulltext
8564 u https://www.mdpi.com/2073-4409/10/6/1543/pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-196280
8564 8u https://doi.org/10.3390/cells10061543
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:146991633

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