Sökning: onr:"swepub:oai:gup.ub.gu.se/314708" > Ribonucleotides emb...
Fältnamn | Indikatorer | Metadata |
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000 | 04112naa a2200517 4500 | |
001 | oai:gup.ub.gu.se/314708 | |
003 | SwePub | |
008 | 240528s2022 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/3147082 URI |
024 | 7 | a https://doi.org/10.1093/nar/gkab12512 DOI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Singh, Meenakshiu Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xsinme |
245 | 1 0 | a Ribonucleotides embedded in template DNA impair mitochondrial RNA polymerase progression |
264 | c 2022-01-08 | |
264 | 1 | b Oxford University Press (OUP),c 2022 |
520 | a Human mitochondria lack ribonucleotide excision repair pathways, causing misincorporated ribonucleotides (rNMPs) to remain embedded in the mitochondrial genome. Previous studies have demonstrated that human mitochondrial DNA polymerase γ can bypass a single rNMP, but that longer stretches of rNMPs present an obstacle to mitochondrial DNA replication. Whether embedded rNMPs also affect mitochondrial transcription has not been addressed. Here we demonstrate that mitochondrial RNA polymerase elongation activity is affected by a single, embedded rNMP in the template strand. The effect is aggravated at stretches with two or more consecutive rNMPs in a row and cannot be overcome by addition of the mitochondrial transcription elongation factor TEFM. Our findings lead us to suggest that impaired transcription may be of functional relevance in genetic disorders associated with imbalanced nucleotide pools and higher levels of embedded rNMPs. © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a DNA | |
653 | a DNA directed DNA polymerase gamma | |
653 | a ribonucleotide | |
653 | a DNA replication | |
653 | a Escherichia coli | |
653 | a genetics | |
653 | a human | |
653 | a metabolism | |
653 | a mitochondrion | |
653 | a DNA Polymerase gamma | |
653 | a Humans | |
653 | a Mitochondria | |
653 | a Ribonucleotides | |
653 | a RNA | |
653 | a Mitochondrial | |
700 | 1 | a Posse, Viktoru Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xposvi |
700 | 1 | a Peter, Bradleyu Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xbradp |
700 | 1 | a Falkenberg, Maria,d 1968u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xfamar |
700 | 1 | a Gustafsson, Claes M,d 1966u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xgucla |
710 | 2 | a Göteborgs universitetb Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi4 org |
773 | 0 | t Nucleic Acids Researchd : Oxford University Press (OUP)g 50:2, s. 989-999q 50:2<989-999x 1362-4962x 0305-1048 |
856 | 4 | u https://academic.oup.com/nar/article-pdf/50/2/989/42269868/gkab1251.pdf |
856 | 4 8 | u https://gup.ub.gu.se/publication/314708 |
856 | 4 8 | u https://doi.org/10.1093/nar/gkab1251 |
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