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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03605naa a2200445 4500 | |
| 001 | oai:research.chalmers.se:ec588cbb-70fc-472d-b8ad-cdc32c1fd684 | |
| 003 | SwePub | |
| 008 | 220609s2022 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://research.chalmers.se/publication/5307452 URI |
| 024 | 7 | a https://doi.org/10.3390/molecules270930222 DOI |
| 040 | a (SwePub)cth | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Zhang, Jingu Tianjin University4 aut |
| 245 | 1 0 | a Adaptive Laboratory Evolution of Halomonas bluephagenesis Enhances Acetate Tolerance and Utilization to Produce Poly(3-hydroxybutyrate) |
| 264 | c 2022-05-08 | |
| 264 | 1 | b MDPI AG,c 2022 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Acetate is a promising economical and sustainable carbon source for bioproduction, but it is also a known cell-growth inhibitor. In this study, adaptive laboratory evolution (ALE) with acetate as selective pressure was applied to Halomonas bluephagenesis TD1.0, a fast-growing and contamination-resistant halophilic bacterium that naturally accumulates poly(3-hydroxybutyrate) (PHB). After 71 transfers, the evolved strain, B71, was isolated, which not only showed better fitness (in terms of tolerance and utilization rate) to high concentrations of acetate but also produced a higher PHB titer compared with the parental strain TD1.0. Subsequently, overexpression of acetyl-CoA synthetase (ACS) in B71 resulted in a further increase in acetate utilization but a decrease in PHB production. Through whole-genome resequencing, it was speculated that genetic mutations (single-nucleotide variation (SNV) in phaB, mdh, and the upstream of OmpA, and insertion of TolA) in B71 might contribute to its improved acetate adaptability and PHB production. Finally, in a 5 L bioreactor with intermittent feeding of acetic acid, B71 was able to produce 49.79 g/L PHB and 70.01 g/L dry cell mass, which were 147.2% and 82.32% higher than those of TD1.0, respectively. These results highlight that ALE provides a reliable method to harness H. bluephagenesis to metabolize acetate for the production of PHB or other high-value chemicals more efficiently. | |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Kemiteknikx Kemiska processer0 (SwePub)204012 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Chemical Engineeringx Chemical Process Engineering0 (SwePub)204012 hsv//eng |
| 650 | 7 | a TEKNIK OCH TEKNOLOGIERx Kemiteknikx Annan kemiteknik0 (SwePub)204992 hsv//swe |
| 650 | 7 | a ENGINEERING AND TECHNOLOGYx Chemical Engineeringx Other Chemical Engineering0 (SwePub)204992 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
| 653 | a PHB | |
| 653 | a adaptive laboratory evolution | |
| 653 | a Halomonas bluephagenesis | |
| 653 | a acetate | |
| 700 | 1 | a Jin, Biaou Tianjin University4 aut |
| 700 | 1 | a Fu, Jing,d 1985u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)jingf |
| 700 | 1 | a Wang, Zhiwenu Tianjin University4 aut |
| 700 | 1 | a Chen, Taou Tianjin University4 aut |
| 710 | 2 | a Tianjin Universityb Chalmers tekniska högskola4 org |
| 773 | 0 | t Moleculesd : MDPI AGg 27:9q 27:9x 1420-3049x 1420-3049 |
| 856 | 4 | u https://research.chalmers.se/publication/530745/file/530745_Fulltext.pdfx primaryx freey FULLTEXT |
| 856 | 4 8 | u https://research.chalmers.se/publication/530745 |
| 856 | 4 8 | u https://doi.org/10.3390/molecules27093022 |
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