Microextraction in Packed Syringe Online with Liquid Chromatography-Tandem Mass Spectrometry: Molecularly imprinted polymer as packing material for MEPS in selective extraction of ropivacaine from plasma

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Fältnamn	Indikatorer	Metadata
000		03521naa a2200409 4500
001		oai:DiVA.org:kau-21457
003		SwePub
008		130121s2006 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-214572 URI
024	7	a https://doi.org/10.1080/108260706007168432 DOI
040		a (SwePub)kau
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Abdel-Rehim, Mohamedu AstraZeneca R&D Södertälje, Södertälje, Sweden4 aut
245	1 0	a Microextraction in Packed Syringe Online with Liquid Chromatography-Tandem Mass Spectrometry :b Molecularly imprinted polymer as packing material for MEPS in selective extraction of ropivacaine from plasma
264		c 2007-02-07
264	1	b Informa UK Limited,c 2006
338		a print2 rdacarrier
520		a The excellent performance of a new sample preparation method, microextraction in packed syringe (MEPS), was recently illustrated by online LC‐MS and GS‐MS assays of local anaesthetics in plasma samples. In the method, approximately 1 mg of solid packing material was inserted into a syringe (100–250 µL) as a plug. Sample preparation took place on the packed bed. The new method was easy to use, fully automated, of low cost, and rapid in comparison with previously used methods. This paper presents the use of molecularly imprinted polymers (MIPs) as packing material for higher extraction selectivity. Development and validation of a method for MIP‐MEPS online with LC‐MS‐MS using ropivacaine in plasma as model compound were investigated. A bupivacaine imprinted polymer was used. The method was validated and the standard curves were evaluated by means of quadratic regression and weighted by inverse of the concentration: 1/x for the calibration range 2–2000 nM. The applied polymer could be used more than 100 times before the syringe was discarded. The extraction recovery was 60%. The results showed high correlation coefficients (R 2 >0.999) for all runs. The accuracy, given as a percentage deviation from the nominal concentration values, ranged from -6% to 3%. The precision, given as the relative standard deviation, at three different concentrations (QC samples) was consistently about 3% to 10%. The limit of quantification was 2 nM.
650	7	a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe
650	7	a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng
653		a Column liquid chromatography‐tandem mass spectrometry
653		a Column liquid chromatography-tandem mass spectrometry
653		a Molecularly imprinted polymers
653		a Ropivacaine
653		a Sample preparation
653		a Chemistry
653		a Kemi
700	1	a Andersson, L.I.u AstraZeneca R&D Södertälje, Södertälje, Sweden4 aut
700	1	a Altun, Zekiu Karlstads universitet,Avdelningen för kemi och biomedicinsk vetenskap4 aut0 (Swepub:kau)zekialtu
700	1	a Blomberg, Lars Gu Karlstads universitet,Avdelningen för kemi och biomedicinsk vetenskap4 aut0 (Swepub:kau)larsblom
710	2	a AstraZeneca R&D Södertälje, Södertälje, Swedenb Avdelningen för kemi och biomedicinsk vetenskap4 org
773	0	t Journal of Liquid Chromatography & Related Technologiesd : Informa UK Limitedg 29:12, s. 1725-1736q 29:12<1725-1736x 1082-6076x 1520-572X
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-21457
856	4 8	u https://doi.org/10.1080/10826070600716843

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Av författaren/redakt...: Abdel-Rehim, Moh ...; Andersson, L.I.; Altun, Zeki; Blomberg, Lars G

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Av lärosätet: Karlstads universitet

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