Modulation of Escherichia coli Cell Membrane by a Monotopic Lipid Glycosyltransferase - an Exploration of Potential Mechanisms

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Fältnamn	Indikatorer	Metadata
000		02715nam a2200313 4500
001		oai:DiVA.org:su-62031
003		SwePub
008		110907\| \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-620312 URI
040		a (SwePub)su
041		a engb eng
042		9 SwePub
072	7	a vet2 swepub-contenttype
072	7	a ovr2 swepub-publicationtype
100	1	a Ge, Changrong,d 1980-u Stockholms universitet,Institutionen för biokemi och biofysik,Åke Wieslander4 aut0 (Swepub:su)chge7872
245	1 0	a Modulation of Escherichia coli Cell Membrane by a Monotopic Lipid Glycosyltransferase - an Exploration of Potential Mechanisms
338		a print2 rdacarrier
520		a Intracellular vesicles are abundant in eukaryotic cells but are rare in Gram-negative bacterium Escherichia coli. Strongly overexpression of a monotopic glycolipid-synthesizing enzyme could induce massive formation of “foreign” vesicles in the cytoplasm. Here we investigate how this membrane-associated enzyme is able to bend and deform the plasma membrane. Limited proteolysis combined with ESI-MS suggested interface binding is mediated through both its two Rossmann fold topological domains. Detailed subcellular localization and liposome binding assay indicates different interface anchoring regions in the protein, and anionic lipid seems to influence the binding properties of the anchoring segments. Genetic engineering of a known membrane-bound segment to explore its vesiculation potentials led to the identification of important catalytic residues (regions). Flow cytometry and infrared spectroscopy were also performed on bacterial cells to get more insight into the cellular morphology and internal complexity. The linking region between two domains was demonstrated to be crucial for both catalytic function and vesiculation capacity of the enzyme. Based on our findings, we propose, that scaffold-like structural feature of this enzyme is most likey one of key elements contributing to vesiculation.
650	7	a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653		a Biochemistry
653		a Biokemi
653		a Biochemistry
653		a biokemi
700	1	a Raussens, Vincent4 aut
700	1	a Ruysschaert, Jean-Marie4 aut
700	1	a Wieslander, Åkeu Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)awies
710	2	a Stockholms universitetb Institutionen för biokemi och biofysik4 org
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-62031

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Av författaren/redakt...: Ge, Changrong, 1 ...; Raussens, Vincen ...; Ruysschaert, Jea ...; Wieslander, Åke

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Biokemi och mole ...

Av lärosätet: Stockholms universitet

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