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Cathelicidin LL-37 ...
Cathelicidin LL-37 and HSV-1 Corneal Infection : Peptide Versus Gene Therapy
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- Lee, Chyan-Jang (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Integrative Regenerative Medicine Centre
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- Buznyk, Oleksiy (författare)
- Filatov Institute of Eye Diseases and Tissue Therapy, Odessa, Ukraine
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- Kuffova, Lucia (författare)
- University of Aberdeen, Scotland
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- Rajendran, Vijayalakshmi (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,University of Aberdeen, Scotland,Integrative Regenerative Medicine Centre
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- Forrester, John V. (författare)
- University of Aberdeen, Scotland
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- Phopase, Jaywant (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Integrative Regenerative Medicine Centre
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- Islam, Mohammad Mirazul (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Integrative Regenerative Medicine Centre
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- Skog, Mårten (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Integrative Regenerative Medicine Centre
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- Ahlqvist, Jenny (författare)
- Vironova AB, Stockholm, Sweden
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- Griffith, May (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Integrative Regenerative Medicine Centre
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(creator_code:org_t)
- Association for Research in Vision and Ophthalmology, 2014
- 2014
- Engelska.
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Ingår i: Translational Vision Science & Technology. - : Association for Research in Vision and Ophthalmology. - 2164-2591. ; 3:3, s. 1-14
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Purpose: To evaluate the potential utility of collagen-based corneal implants with anti?Herpes Simplex Virus (HSV)-1 activity achieved through sustained release of LL-37, from incorporated nanoparticles, as compared with cell-based delivery from model human corneal epithelial cells (HCECs) transfected to produce endogenous LL-37. Methods: We tested the ability of collagen-phosphorylcholine implants to tolerate the adverse microenvironment of herpetic murine corneas. Then, we investigated the efficacy of LL-37 peptides delivered through nanoparticles incorporated within the corneal implants to block HSV-1 viral activity. In addition, LL-37 complementary DNA (cDNA) was transferred into HCECs to confer viral resistance, and their response to HSV-1 infection was examined. Results: Our implants remained in herpetic murine corneas 7 days longer than allografts. LL-37 released from the implants blocked HSV-1 infection of HCECs by interfering with viral binding. However, in pre-infected HCECs, LL-37 delayed but could not prevent viral spreading nor clear viruses from the infected cells. HCECs transfected with the LL-37 expressed and secreted the peptide. Secreted LL-37 inhibited viral binding in vitro but was insufficient to protect cells completely from HSV-1 infection. Nevertheless, secreted LL-37 reduced both the incidence of plaque formation and plaque size. Conclusion: LL-37 released from composite nanoparticle-hydrogel corneal implants and HCEC-produced peptide, both showed anti?HSV-1 activity by blocking binding. However, while both slowed down virus spread, neither was able on its own to completely inhibit the viruses. Translational Relevance: LL-37 releasing hydrogels may have potential utility as corneal substitutes for grafting in HSV-1 infected corneas, possibly in combination with LL-37 producing therapeutic cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
Nyckelord
- cornea
- HSV-1
- antiviral peptides; nanoparticles
- gene transfer
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Lee, Chyan-Jang
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Buznyk, Oleksiy
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Kuffova, Lucia
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Rajendran, Vijay ...
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Forrester, John ...
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Phopase, Jaywant
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Islam, Mohammad ...
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Skog, Mårten
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Ahlqvist, Jenny
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Griffith, May
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Oftalmologi
- Artiklar i publikationen
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Translational Vi ...
- Av lärosätet
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Linköpings universitet