Primary structure of guinea pig preprodynorphin and preproenkephalin mRNAs: multiple transcription initiation sites for preprodynorphin

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: WFRF:(Steven K) > (2000-2004) > Primary structure o...

LIBRIS Formathandbok (Information om MARC21)

Fältnamn	Indikatorer	Metadata
000		02846naa a2200277 4500
001		oai:DiVA.org:uu-91413
003		SwePub
008		040311s2004 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-914132 URI
024	7	a https://doi.org/10.1016/j.brainresbull.2004.01.0082 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a LaForge, K. Stevenu Uppsala universitet,Avdelningen för biologisk beroendeforskning4 aut
245	1 0	a Primary structure of guinea pig preprodynorphin and preproenkephalin mRNAs :b multiple transcription initiation sites for preprodynorphin
264	1	b Elsevier BV,c 2004
338		a print2 rdacarrier
520		a Preprodynorphin and preproenkephalin are protein precursors from which are derived two classes of opioid neurotransmitter peptides. Dynorphin A((1-17)) is produced by proteolytic processing of prodynorphin, and processing of proenkephalin yields the enkephalin peptides. We report here on the isolation and sequencing of multiple clones for these two mRNAs from a cDNA library. Two cDNA clones of preprodynorphin contained the full-length sequence (2.35 kb) with the primary structure predicted from the guinea pig gene sequence. In contrast, one clone encoded the full-length sequence but also an additional 192 nt at the 5' end. This sequence has high homology to the 5' flanking region of the human preprodynorphin gene, and RNase protection assays demonstrated that in addition to a primary initiation site, transcription of this mRNA is initiated at several sites 160-190 nt 5' with respect to the primary site. This difference may alter translational efficiency or mRNA stability. The sequence of preproenkephalin cDNA clones confirmed the structure predicted from the gene sequence. One clone, however, contained sequences encoded by exons 2 and 3, and initiated within the first intron (intron A) of the gene. We used RNase protection mapping to assess the abundance in the brain and pituitary of preproenkephalin transcripts that initiate within intron A. These studies confirmed that the primary transcription start site is 28 nucleotides downstream from the TATAA site, and that intron A sequences are not present in significant amounts in these tissues.
700	1	a Nyberg, Fredu Uppsala universitet,Institutionen för farmaceutisk biovetenskap4 aut0 (Swepub:uu)frednybe
700	1	a Kreek, Mary Jeanne4 aut
710	2	a Uppsala universitetb Avdelningen för biologisk beroendeforskning4 org
773	0	t Brain Research Bulletind : Elsevier BVg 63:2, s. 119-126q 63:2<119-126x 0361-9230x 1873-2747
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-91413
856	4 8	u https://doi.org/10.1016/j.brainresbull.2004.01.008

Hitta via bibliotek

Brain Research Bulletin (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: LaForge, K. Stev ...; Nyberg, Fred; Kreek, Mary Jean ...

Artiklar i publikationen: Brain Research B ...

Av lärosätet: Uppsala universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se