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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004208naa a2200493 4500
001oai:DiVA.org:uu-472395
003SwePub
008220420s2022 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4723952 URI
0247 a https://doi.org/10.1016/j.fawpar.2021.e001422 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Ahlinder, J.u FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.4 aut
2451 0a Use of metagenomic microbial source tracking to investigate the source of a foodborne outbreak of cryptosporidiosis
264 1b Elsevier,c 2022
338 a electronic2 rdacarrier
520 a Cryptosporidium is a protozoan parasite of global public health importance that causes gastroenteritis in a variety of vertebrate hosts, with many human outbreaks reported yearly, often from ingestion of contaminated water or food. Despite the major public health implications, little is typically known about sources of contamination of disease outbreaks caused by Cryptosporidium. Here, we study a national foodborne outbreak resulted from infection with Cryptosporidium parvum via romaine lettuce, with the main goal to trace the source of the parasite. To do so, we combined traditional outbreak investigation methods with molecular detection and characterization methods (i.e. PCR based typing, amplicon and shotgun sequencing) of romaine lettuce samples collected at the same farm from which the contaminated food was produced. Using 18S rRNA typing, we detected C. parvum in two out of three lettuce samples, which was supported by detections in the metagenome analysis. Microbial source tracking analysis of the lettuce samples suggested sewage water as a likely source of the contamination, albeit with some uncertainty. In addition, the high degree of overlap in bacterial species content with a public human gut microbial database corroborated the source tracking results. The combination of traditional and molecular based methods applied here is a promising tool for future source tracking investigations of food- and waterborne outbreaks of Cryptosporidium spp. and can help to control and mitigate contamination risks.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng
653 a Vegetables
653 a Contaminated food
653 a Cryptosporidium parvum
653 a Foodborne outbreak investigation
653 a 18S rRNA
653 a 16S rRNA
7001 a Svedberg, A-Lu Reg Norrbotten, Dept Infect Control, Luleå, Sweden.4 aut
7001 a Nystedt, A.u Reg Norrbotten, Dept Infect Control, Luleå, Sweden.4 aut
7001 a Dryselius, R.u Natl Food Agcy, Dept Biol, Uppsala, Sweden.4 aut
7001 a Jacobsson, K.u Natl Food Agcy, Dept Biol, Uppsala, Sweden.4 aut
7001 a Hägglund, M.u Clin Genom Facil, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.4 aut
7001 a Brindefalk, B.u FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.4 aut
7001 a Forsman, M.u FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.4 aut
7001 a Ottoson, J.u Natl Food Agcy, Dept Risk & Benefit Assessment, Uppsala, Sweden.4 aut
7001 a Troell, Karinu Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Natl Vet Inst, Dept Microbiol, Uppsala, Sweden.4 aut0 (Swepub:uu)kartr688
7102 a FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.b Reg Norrbotten, Dept Infect Control, Luleå, Sweden.4 org
7730 t FOOD AND WATERBORNE PARASITOLOGYd : Elsevierg 26q 26x 2405-6766
8564 u https://doi.org/10.1016/j.fawpar.2021.e00142y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1652792/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://doi.org/10.1016/j.fawpar.2021.e00142
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-472395
8564 8u https://doi.org/10.1016/j.fawpar.2021.e00142

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