SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Emilsson Tobias)
 

Sökning: WFRF:(Emilsson Tobias) > (2015-2019) > Rapid identificatio...

  • Nyberg, Lena,1979Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden (författare)

Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules

  • Artikel/kapitelEngelska2016

Förlag, utgivningsår, omfång ...

  • 2016-07-27
  • Springer Science and Business Media LLC,2016
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:research.chalmers.se:02e13c61-d0ca-4e3c-bf28-c4f500698ac3
  • https://research.chalmers.se/publication/240821URI
  • https://doi.org/10.1038/srep30410DOI
  • https://lup.lub.lu.se/record/335b6cd4-e144-4704-a953-bf213ad5decbURI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-308254URI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17700URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:art swepub-publicationtype
  • Ämneskategori:ref swepub-contenttype

Anmärkningar

  • The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of antibiotic resistance. Toward this aim, we have developed an optical DNA mapping procedure where individual intact plasmids are elongated within nanofluidic channels and visualized through fluorescence microscopy, yielding barcodes that reflect the underlying sequence. The assay rapidly identifies plasmids through statistical comparisons with barcodes based on publicly available sequence repositories and also enables detection of structural variations. Since the assay yields holistic sequence information for individual intact plasmids, it is an ideal complement to next generation sequencing efforts which involve reassembly of sequence reads from fragmented DNA molecules. The assay should be applicable in microbiology labs around the world in applications ranging from fundamental plasmid biology to clinical epidemiology and diagnostics.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Quaderi, Mahmood Saair,1990Chalmers University of Technology,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden / Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.;Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.(Swepub:cth)saair (författare)
  • Emilsson, Gustav,1989Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.;Chalmers, Dept Appl Phys, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden / Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden(Swepub:cth)gusemi (författare)
  • Karami, N.University of Gothenburg,Univ Gothenburg, Sahlgrenska Acad, Dept Infect Dis, Gothenburg, Sweden.,Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden (författare)
  • Lagerstedt, ErikLunds universitet,Lund University,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.(Swepub:his)lagf (författare)
  • Müller, Vilhelm,1990Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden(Swepub:cth)mullerv (författare)
  • Noble, CharlestonLund University,Lunds universitet,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden(Swepub:lu)thep-can (författare)
  • Hammarberg, S.Lunds universitet,Lund University,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden. (författare)
  • Nilsson, Adam N.Lund University,Lunds universitet,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden(Swepub:lu)fysi-adn (författare)
  • Sjoberg, F.University of Gothenburg,Univ Gothenburg, Sahlgrenska Acad, Dept Infect Dis, Gothenburg, Sweden.,Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden (författare)
  • Fritzsche, Joachim,1977Chalmers University of Technology,Chalmers, Dept Appl Phys, Gothenburg, Sweden.,Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden(Swepub:cth)joafri (författare)
  • Sandegren, LinusUppsala universitet,Uppsala University,Institutionen för medicinsk biokemi och mikrobiologi,Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden(Swepub:uu)lisan839 (författare)
  • Ambjörnsson, TobiasLund University,Lunds universitet,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden(Swepub:lu)thep-tar (författare)
  • Westerlund, Fredrik,1978Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden(Swepub:cth)fredrikw (författare)
  • Kristiansson, Erik,1978University of Gothenburg,Chalmers University of Technology,Univ Gothenburg, Dept Math Sci, Chalmers Univ Technol, Gothenburg, Sweden.,Department of Mathematical Sciences, Chalmers University of Technology, University of Gothenburg, Gothenburg, Sweden(Swepub:cth)erikkr (författare)
  • Chalmers University of TechnologyChalmers, Dept Biol & Biol Engn, Gothenburg, Sweden. (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Scientific Reports: Springer Science and Business Media LLC62045-23222045-2322

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy