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  • Liu, Kang-ChengUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Umeå Univ, Integrat Med Biol, S-90187 Umeå, Sweden. (author)

Membrane insertion mechanism of the caveola coat protein Cavin1

  • Article/chapterEnglish2022

Publisher, publication year, extent ...

  • 2022-06-13
  • Proceedings of the National Academy of Sciences,2022
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:umu-203198
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-203198URI
  • https://doi.org/10.1073/pnas.2202295119DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-482694URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Caveolae are small plasma membrane invaginations, important for control of membrane tension, signaling cascades, and lipid sorting. The caveola coat protein Cavin1 is essential for shaping such high curvature membrane structures. Yet, a mechanistic understanding of how Cavin1 assembles at the membrane interface is lacking. Here, we used model membranes combined with biophysical dissection and computational modeling to show that Cavin1 inserts into membranes. We establish that initial phosphatidylinositol (4, 5) bisphosphate [PI(4,5)P2]-dependent membrane adsorption of the trimeric helical region 1 (HR1) of Cavin1 mediates the subsequent partial separation and membrane insertion of the individual helices. Insertion kinetics of HR1 is further enhanced by the presence of flanking negatively charged disordered regions, which was found important for the coassembly of Cavin1 with Caveolin1 in living cells. We propose that this intricate mechanism potentiates membrane curvature generation and facilitates dynamic rounds of assembly and disassembly of Cavin1 at the membrane.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Pace, HudsonUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Umeå Univ, Integrat Med Biol, S-90187 Umeå, Sweden.;Umeå Univ, Dept Clin Microbiol, S-90185 Umeå, Sweden.;Umeå Univ, Wallenberg Ctr Mol Med, S-90185 Umeå, Sweden.(Swepub:umu)hupa0005 (author)
  • Larsson, ElinUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Medicinsk och klinisk genetik,Institutionen för medicinsk kemi och biofysik,Umeå Univ, Integrat Med Biol, S-90187 Umeå, Sweden.(Swepub:umu)elnlan01 (author)
  • Hossain, Md ShakhawathUppsala universitet,Institutionen för farmaci(Swepub:uu)mdho258 (author)
  • Kabedev, AlekseiUppsala universitet,Institutionen för farmaci(Swepub:uu)aleka155 (author)
  • Shukla, AnkitaUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Umeå Univ, Integrat Med Biol, S-90187 Umeå, Sweden.(Swepub:umu)ansh0030 (author)
  • Jerschabek, VanessaInstitute of Physical Chemistry, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany,Martin Luther Univ Halle Wittenberg, Inst Phys Chem, D-06120 Halle, Saale, Germany. (author)
  • Mohan, JaganUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Umeå Univ, Integrat Med Biol, S-90187 Umeå, Sweden.;Inst Pasteur, Membrane Biochem & Transport, F-75015 Paris, France.(Swepub:umu)jamo0018 (author)
  • Bergström, Christel,1973-Uppsala universitet,Institutionen för farmaci(Swepub:uu)cjo29958 (author)
  • Bally, MartaUmeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Umeå Centre for Microbial Research (UCMR),Umeå Univ, Dept Clin Microbiol, S-90185 Umeå, Sweden.;Umeå Univ, Wallenberg Ctr Mol Med, S-90185 Umeå, Sweden.(Swepub:umu)maba0261 (author)
  • Schwieger, ChristianInstitute of Physical Chemistry, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany,Martin Luther Univ Halle Wittenberg, Inst Phys Chem, D-06120 Halle, Saale, Germany. (author)
  • Hubert, MadlenUppsala universitet,Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Department of Pharmacy, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden,Institutionen för farmaci,Umeå Univ, Integrat Med Biol, S-90187 Umeå, Sweden.(Swepub:uu)madhu517 (author)
  • Lundmark, RichardUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Institutionen för medicinsk kemi och biofysik,Molekylär Infektionsmedicin, Sverige (MIMS),Umeå Univ, Integrat Med Biol, S-90187 Umeå, Sweden.(Swepub:umu)rilu0004 (author)
  • Umeå universitetInstitutionen för integrativ medicinsk biologi (IMB) (creator_code:org_t)

Related titles

  • In:Proceedings of the National Academy of Sciences of the United States of America: Proceedings of the National Academy of Sciences119:250027-84241091-6490

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