Sökning: WFRF:(Kriz Vitezslav) > Temporal Dynamics o...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03918naa a2200517 4500 | |
001 | oai:DiVA.org:uu-400532 | |
003 | SwePub | |
008 | 191223s2019 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4005322 URI |
024 | 7 | a https://doi.org/10.3390/cells81216452 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Pietilä, Ilkkau Uppsala universitet,Institutionen för medicinsk cellbiologi,Vaskulärbiologi4 aut0 (Swepub:uu)ilkpi999 |
245 | 1 0 | a Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB |
264 | c 2019-12-15 | |
264 | 1 | a Basel, Switzerland :b MDPI,c 2019 |
338 | a electronic2 rdacarrier | |
520 | a Focal adhesion kinase (FAK) is essential for vascular endothelial growth factor-A (VEGFA)/VEGF receptor-2 (VEGFR2)-stimulated angiogenesis and vascular permeability. We have previously noted that presence of the Src homology-2 domain adapter protein B (SHB) is of relevance for VEGFA-stimulated angiogenesis in a FAK-dependent manner. The current study was conducted in order address the temporal dynamics of co-localization between these components in HEK293 and primary lung endothelial cells (EC) by total internal reflection fluorescence microscopy (TIRF). An early (<2.5 min) VEGFA-induced increase in VEGFR2 co-localization with SHB was dependent on tyrosine 1175 in VEGFR2. VEGFA also enhanced SHB co-localization with FAK. FAK co-localization with VEGFR2 was dependent on SHB since it was significantly lower in SHB deficient EC after VEGFA addition. Absence of SHB also resulted in a gradual decline of VEGFR2 co-localization with FAK under basal (prior to VEGFA addition) conditions. A similar basal response was observed with expression of the Y1175F-VEGFR2 mutant in wild type EC. The distribution of focal adhesions in SHB-deficient EC was altered with a primarily perinuclear location. These live cell data implicate SHB as a key component regulating FAK activity in response to VEGFA/VEGFR2. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaper0 (SwePub)3012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicine0 (SwePub)3012 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Cellbiologi0 (SwePub)106042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Cell Biology0 (SwePub)106042 hsv//eng |
653 | a VEGFR2 | |
653 | a FAK | |
653 | a SHB | |
653 | a TIRF | |
653 | a focal adhesions | |
653 | a angiogenesis | |
653 | a Molekylär cellbiologi | |
653 | a Molecular Cellbiology | |
700 | 1 | a van Mourik, Djenolanu Uppsala universitet,Institutionen för medicinsk cellbiologi4 aut |
700 | 1 | a Tamelander, Andreasu Uppsala universitet,Institutionen för medicinsk cellbiologi4 aut |
700 | 1 | a Kriz, Vitezslavu Institute of Molecular Genetics of the CAS, 14220 Prague, Czech Republic4 aut |
700 | 1 | a Claesson-Welsh, Lenau Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)lcl04207 |
700 | 1 | a Tengholm, Anders,d 1971-u Uppsala universitet,Institutionen för medicinsk cellbiologi4 aut0 (Swepub:uu)andeteng |
700 | 1 | a Welsh, Michael,d 1957-u Uppsala universitet,Institutionen för medicinsk cellbiologi4 aut0 (Swepub:uu)michwels |
710 | 2 | a Uppsala universitetb Institutionen för medicinsk cellbiologi4 org |
773 | 0 | t Cellsd Basel, Switzerland : MDPIg 8:12q 8:12x 2073-4409 |
856 | 4 | u https://doi.org/10.3390/cells8121645y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1381576/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://www.mdpi.com/2073-4409/8/12/1645/pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-400532 |
856 | 4 8 | u https://doi.org/10.3390/cells8121645 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
Kopiera och spara länken för att återkomma till aktuell vy