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Phosphoproteomic in...
Phosphoproteomic insights into processes influenced by the kinase-like protein DIA1/C3orf58
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- Hareza, Agnieszka (författare)
- International Institute of Molecular and Cell Biology, Warsaw,Warsaw University of Life Sciences
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- Bakun, Magda (författare)
- Institute of Biochemistry and Biophysics of the Polish Academy of Sciences
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- Świderska, Bianka (författare)
- Institute of Biochemistry and Biophysics of the Polish Academy of Sciences
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- Dudkiewicz, Małgorzata (författare)
- Warsaw University of Life Sciences
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- Koscielny, Alicja (författare)
- International Institute of Molecular and Cell Biology, Warsaw
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- Bajur, Anna (författare)
- Warsaw University of Life Sciences,International Institute of Molecular and Cell Biology, Warsaw,Max Planck Institute of Molecular Cell Biology and Genetics
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- Jaworski, Jacek (författare)
- International Institute of Molecular and Cell Biology, Warsaw
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- Dadlez, Michał (författare)
- Institute of Biochemistry and Biophysics of the Polish Academy of Sciences
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- Pawłowski, Krzysztof (författare)
- Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine,Warsaw University of Life Sciences
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(creator_code:org_t)
- 2018-04-09
- 2018
- Engelska.
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Ingår i: PeerJ. - : PeerJ. - 2167-8359. ; 2018:4
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://doi.org/10.7...
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https://lup.lub.lu.s...
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https://doi.org/10.7...
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Abstract
Ämnesord
Stäng
- Many kinases are still 'orphans,' which means knowledge about their substrates, and often also about the processes they regulate, is lacking. Here, DIA1/C3orf58, a member of a novel predicted kinase-like family, is shown to be present in the endoplasmic reticulum and to influence trafficking via the secretory pathway. Subsequently, DIA1 is subjected to phosphoproteomics analysis to cast light on its signalling pathways. A liquid chromatography-tandem mass spectrometry proteomic approach with phosphopeptide enrichment is applied to membrane fractions of DIA1-overexpressing and control HEK293T cells, and phosphosites dependent on the presence of DIA1 are elucidated. Most of these phosphosites belonged to CK2- and proline-directed kinase types. In parallel, the proteomics of proteins immunoprecipitated with DIA1 reported its probable interactors. This pilot study provides the basis for deeper studies of DIA1 signalling.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Mass spectrometry
- Novel kinases
- Phosphoproteomics
- Secretory pathway
- Signalling
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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