Universal Biomaterial-on-Chip: A modular platform for flexible biomaterial integration and versatile quantitative assessment

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Sökning: WFRF:(Atif Abdul Raouf 1996 ) > Universal Biomateri...

Atif, Abdul Raouf,1996-Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Division of Biomedical Engineering, EMBLA group (författare)

Universal Biomaterial-on-Chip: A modular platform for flexible biomaterial integration and versatile quantitative assessment

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LIBRIS-ID:oai:DiVA.org:uu-497277
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-497277URI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:vet swepub-contenttype
Ämneskategori:ovr swepub-publicationtype

Anmärkningar

A requirement for clinical approval is verification of a biomaterial’s functionality and biocompatibility. However, discrepancies between in vitro and in vivo evaluations have been reported, possibly due in part to a lack of physiological relevance of typical in vitro culture set-ups. We introduce a Universal Biomaterial-on-Chip (UBoC), which is a microfluidics device that allows integration of biomaterials with varied shapes and properties and subsequent evaluation of in vitro performance under design considerations that resemble physiological conditions. In addition, UBoC operates with multifunctional modalities such as continuous perfusion, shear stress mechanostimulation and cell co-culture. The device is constructed using simple 3D printing and microfabrication techniques and its cell culture area resembles a 96-well plate (0.32 cm2). Successful cell adhesion and proliferation was observed on-chip on different materials (hydroxyapatite, titanium and fibrin) using fluorescence microscopy. Furthermore, device applicability for mechanostimulation was demonstrated through shear stimulation, where sensitivity of pre-osteoblasts to flow was captured via live Ca2+ imaging. Finally, the modularity of the UBoC platform for on-chip co-culture experiments was established after simple modifications of on-board fluidic arrangements. Overall, the UBoC presents a useful tool that augments existing in vitro testing strategies and enables thorough comparisons between biomaterials in tunable culture conditions.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology hsv//eng
Biomaterials
Calcium imaging
Mechanobiology
Microfluidics
Standardization
3D printing
Engineering Science with specialization in Biomedical Engineering
Teknisk fysik med inriktning mot medicinsk teknik

Biuppslag (personer, institutioner, konferenser, titlar ...)

Aramesh, MortezaUppsala universitet,Institutionen för materialvetenskap,Division of Biomedical Engineering, BMS group(Swepub:uu)morar401 (författare)
Carter, Sarah-Sophia,1994-Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Division of Biomedical Engineering, EMBLA group(Swepub:uu)sarca385 (författare)
Tenje, MariaUppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Division of Biomedical Engineering, EMBLA group(Swepub:uu)marte323 (författare)
Mestres, Gemma,1984-Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik,Division of Biomedical Engineering, EMBLA group(Swepub:uu)gemme924 (författare)
Uppsala universitetMikrosystemteknik (creator_code:org_t)

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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-497277

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Av författaren/redakt...: Atif, Abdul Raou ...; Aramesh, Morteza; Carter, Sarah-So ...; Tenje, Maria; Mestres, Gemma, ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinsk biotek ...

Av lärosätet: Uppsala universitet

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