Lipid-Based Bioanalytical Sensors

Bally, Marta, 1981 (författare): Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Department of Physics, Chalmers University of Technology, Gothenburg, Sweden,Chalmers tekniska högskola,Chalmers University of Technology,Umeå University

Pace, Hudson, 1982 (författare): Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Chalmers tekniska högskola,Chalmers University of Technology,Umeå University

Höök, Fredrik, 1966 (författare): Chalmers tekniska högskola,Chalmers University of Technology,Department of Physics, Chalmers University of Technology, Gothenburg, Sweden

(creator_code:org_t)

Boca Raton : CRC Press, 2021
2021
Engelska.
Ingår i: Handbook of Lipid Membranes Molecular, Functional, and Materials Aspects. - Boca Raton : CRC Press. - 9781466555730 ; , s. 241-270, s. 241-269

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Abstract Ämnesord

Stäng

Lipid assemblies have attracted considerable interest as components in bioanalytical sensors. They provide a native-like environment for the immobilization of membrane proteins and for the study of membrane-related processes. Liposomes are also excellent bioanalytical assay components since selected functionalities can be added to the membrane while their aqueous interior can encapsulate a variety of molecules. This chapter highlights the potential of lipid assemblies in surface-based affinity sensors. It first describes how such sensors are created, providing an overview of lipid immobilization strategies together with a summary of the major transduction techniques used to probe binding at and transport through membrane interfaces. It then reviews the implementation of lipid-based sensors in the study of membrane proteins and membrane-mediated interactions, followed by a discussion of the potential of liposomes as nanoscale labels and as nanoreactors. Finally, it illustrates how external forces can be used to manipulate membrane component for biosensing applications.

Av författaren/redakt...: Bally, Marta, 19 ...; Pace, Hudson, 19 ...; Höök, Fredrik, 1 ...

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