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PDMS leaching and its implications for on-chip studies focusing on bone regeneration applications
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- Carter, Sarah-Sophia, 1994- (författare)
- Uppsala universitet,Mikrosystemteknik,EMBLA
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- Atif, Abdul Raouf, 1996- (författare)
- Uppsala universitet,Mikrosystemteknik,EMBLA
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- Kadekar, Sandeep (författare)
- Uppsala universitet,Polymerkemi
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- (creator_code:org_t)
- Elsevier, 2020
- 2020
- Engelska.
- Ingår i: Organs-on-a-Chip. - : Elsevier. - 2666-1020. ; 2
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Polydimethylsiloxane (PDMS) is among the most widely used materials for organ-on-chip systems. Despite itsmultiple beneficial characteristics from an engineering point of view, there is a concern about the effect of PDMSon the cells cultured in such devices. The aim of this study was to enhance the understanding of the effect of PDMSon cellular behavior in a context relevant for on-chip studies. The focus was put on an indirect effect of PDMS,namely leaching of uncrosslinked oligomers, particularly for bone regeneration applications. PDMS-based chipswere prepared and analyzed for the potential release of PDMS oligomers within the microfluidic channel whenkept at different flow rates. Leaching of uncrosslinked oligomers from PDMS was quantified as silicon concen-tration by inductively coupled plasma - optical emission spectrometry and further confirmed by mass spec-trometry. Subsequently, PDMS-leached media, with a silicon concentration matching the on-chip experiment,were prepared to study cell proliferation and osteogenic differentiation of MC3T3-E1 pre-osteoblasts and humanmesenchymal stem cells. The silicon concentration initially detected in the media was inversely proportional tothe tested flow rates and decreased to control levels within 52 h. In addition, by curing the material overnightinstead of 2 h, regardless of the curing temperature (65 and 80 C), a large reduction in silicon concentration wasfound, indicating the importance of the PDMS curing parameters. Furthermore, it was shown that PDMS oligo-mers enhanced the differentiation of MC3T3-E1 pre-osteoblasts, this being a cell type dependent effect as nochanges in cell differentiation were observed for human mesenchymal stem cells. Overall, this study illustrates theimportance of optimization steps when using PDMS devices for biological studies, in particular PDMS curingconditions and extensive washing steps prior to an experiment.
Nyckelord
- PDMS
- Organs-on-chip
- Human mesenchymal stem cells
- Osteoblasts
- Silicon
- Teknisk fysik med inriktning mot mikrosystemteknik
- Engineering Science with specialization in Microsystems Technology
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