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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02941naa a2200445 4500 | |
| 001 | oai:gup.ub.gu.se/248477 | |
| 003 | SwePub | |
| 008 | 240528s1995 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://gup.ub.gu.se/publication/2484772 URI |
| 040 | a (SwePub)gu | |
| 041 | a eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Skoglund, Thomas,d 1969u Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology4 aut0 (Swepub:gu)xskoth |
| 245 | 1 0 | a A method for 3D reconstruction of neuronal processes using semithin serial sections displayed as a cinematographic sequence. |
| 264 | 1 | c 1995 |
| 520 | a In order to study the organization and distribution of dendrites and axons in the cerebral cortex, we have developed a computer-assisted method for 3D reconstruction of neuronal processes based on serial light microscopic images displayed as a continuous sequence. A series of tangential sections (0.65 micron thick) through rat parietal cortex was aligned, digitized into the computer and then used to build a sequence (stack) of images which was stored to a digital real-time video disk. Apical dendrites located in dendritic bundles in laminae III and IV were traced through the sequence. Two tracing modes were tested: (1) cinematographic mode, in which the image stack was displayed continuously and automatically by the computer at various preset speeds (max. speed: 25 images/s) and (2) stepping mode, in which the interval between each image was varied manually according to the choice of the operator. Coordinates were stored in a database and used to build a 3D reconstruction where apical dendrites were displayed as wires or tubes. Tracing in cinematographic mode was about 3 times faster than tracing in stepping mode. We believe that the former mode exploits the built in 'filtering' capacity of the visual system to perform temporal averaging. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng |
| 653 | a Animals | |
| 653 | a Axons | |
| 653 | a ultrastructure | |
| 653 | a Cerebral Cortex | |
| 653 | a physiology | |
| 653 | a ultrastructure | |
| 653 | a Dendrites | |
| 653 | a physiology | |
| 653 | a ultrastructure | |
| 653 | a Image Processing | |
| 653 | a Computer-Assisted | |
| 653 | a methods | |
| 653 | a Neural Pathways | |
| 653 | a ultrastructure | |
| 653 | a Rats | |
| 700 | 1 | a Pascher, R4 aut |
| 700 | 1 | a Berthold, C H4 aut |
| 710 | 2 | a Göteborgs universitetb Institutionen för anatomi och cellbiologi4 org |
| 773 | 0 | t Journal of neuroscience methodsg 61:1-2, s. 105-11q 61:1-2<105-11x 0165-0270 |
| 856 | 4 8 | u https://gup.ub.gu.se/publication/248477 |
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