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  • Nilsson, D.Karolinska Institutet (author)

Whole-Genome Sequencing of Cytogenetically Balanced Chromosome Translocations Identifies Potentially Pathological Gene Disruptions and Highlights the Importance of Microhomology in the Mechanism of Formation

  • Article/chapterEnglish2017

Publisher, publication year, extent ...

  • 2016-12-05
  • John Wiley & Sons,2017
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-201964
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-201964URI
  • https://doi.org/10.1002/humu.23146DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-141372URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:135170270URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Funding text: Contract grant sponsors: Swedish Research Council [2012-1526, 2013-2603]; The Swedish Society for Medical Research; the Marianne and Marcus Wallenberg Foundation [2014.0084]; Stockholm County Council; the Harald and Greta Jeanssons Foundation; the Ulf Lundahl memory fund through the Swedish Brain Foundation; the Nilsson Ehle donations and the Erik Rönnberg Foundation; US National Institutes of Health [HG006542] to the Baylor Hopkins Center for Mendelian Genomics. We are grateful to the patients and their families for their cooperation and enthusiasm during this study. We also gratefully acknowledge the use of computer infrastructure resources at UPPMAX, projects b2011162 and b2014152.QC 20170303
  • Most balanced translocations are thought to result mechanistically from nonhomologous end joining or, in rare cases of recurrent events, by nonallelic homologous recombination. Here, we use low-coverage mate pair whole-genome sequencing to fine map rearrangement breakpoint junctions in both phenotypically normal and affected translocation carriers. In total, 46 junctions from 22 carriers of balanced translocations were characterized. Genes were disrupted in 48% of the breakpoints; recessive genes in four normal carriers and known dominant intellectual disability genes in three affected carriers. Finally, seven candidate disease genes were disrupted in five carriers with neurocognitive disabilities (SVOPL, SUSD1, TOX, NCALD, SLC4A10) and one XX-male carrier with Tourette syndrome (LYPD6, GPC5). Breakpoint junction analyses revealed microhomology and small templated insertions in a substantive fraction of the analyzed translocations (17.4%; n = 4); an observation that was substantiated by reanalysis of 37 previously published translocation junctions. Microhomology associated with templated insertions is a characteristic seen in the breakpoint junctions of rearrangements mediated by error-prone replication-based repair mechanisms. Our data implicate that a mechanism involving template switching might contribute to the formation of at least 15% of the interchromosomal translocation events.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Pettersson, M.Karolinska Institutet (author)
  • Gustavsson, P.Karolinska Institutet (author)
  • Förster, A. (author)
  • Hofmeister, W.Karolinska Institutet (author)
  • Wincent, J.Karolinska Institutet (author)
  • Zachariadis, V.Karolinska Institutet (author)
  • Anderlid, B. -MKarolinska Institutet (author)
  • Nordgren, A.Karolinska Institutet (author)
  • Mäkitie, O.Karolinska Institutet (author)
  • Wirta, ValtteriKarolinska Institutet,KTH,Genteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1u59vht (author)
  • Käller, MaxKTH,Genteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u17gryzz (author)
  • Vezzi, FrancescoStockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för biokemi och biofysik(Swepub:su)fvezz (author)
  • Lupski, J. R. (author)
  • Nordenskjöld, M.Karolinska Institutet (author)
  • Syk Lundberg, E.Karolinska Institutet (author)
  • Carvalho, C. M. B. (author)
  • Lindstrand, A.Karolinska Institutet (author)
  • Karolinska InstitutetGenteknologi (creator_code:org_t)

Related titles

  • In:Human Mutation: John Wiley & Sons38:2, s. 180-1921059-77941098-1004

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